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Ache (NM_009599) Mouse Tagged ORF Clone
CAT＃: MR226310
- TrueORF®
Ache (Myc-DDK-tagged) - Mouse acetylcholinesterase (Ache)
ORF Plasmid: tGFP
"NM_009599" in other vectors (4)
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CNY 6,856.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mE1a; mE1b; mE1c; mE1c-long; mE1d; mE1d'; mE1e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226310 representing NM_009599
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCTCCCTGGTATCCCCTGCATACACCTTCCCTGGCTTTTCCACTCCTCTTCCTCCTCCTCTCCC TCCTGGGAGGAGGGGCAAGGGCTGAGGGCCGGGAAGACCCGCAGCTGCTGGTGAGGGTTCGAGGGGGCCA GCTGAGGGGCATCCGCCTGAAGGCCCCTGGAGGCCCAGTCTCAGCTTTTCTGGGCATCCCCTTTGCAGAG CCACCTGTGGGCTCACGTAGATTTATGCCACCAGAGCCCAAGCGGCCCTGGTCAGGAGTGTTGGATGCTA CCACCTTCCAAAATGTCTGCTACCAGTACGTGGACACCCTGTACCCTGGGTTTGAGGGTACTGAGATGTG GAACCCCAACCGAGAGTTGAGTGAAGACTGCCTGTATCTTAATGTGTGGACACCATACCCCAGACCTGCT TCTCCCACACCTGTCCTCATCTGGATCTATGGGGGTGGTTTCTACAGCGGAGCGGCCTCCTTGGATGTGT ATGACGGCCGTTTCCTGGCCCAGGTTGAGGGAGCTGTGTTGGTATCTATGAACTACCGAGTGGGAACCTT TGGCTTCTTGGCCCTACCAGGAAGCAGAGAAGCCCCTGGCAATGTAGGTCTGCTGGATCAACGGCTTGCC TTGCAATGGGTGCAAGAAAATATTGCAGCCTTTGGGGGCGACCCGATGTCAGTGACTCTGTTTGGGGAGA GTGCGGGTGCAGCCTCCGTGGGCATGCACATACTGTCCCTGCCCAGCAGGAGCCTCTTCCACAGGGCTGT CCTCCAGAGTGGCACACCCAATGGGCCCTGGGCCACTGTGAGTGCTGGAGAGGCCAGGCGCAGGGCCACA CTGCTGGCCCGCCTTGTGGGCTGTCCCCCAGGTGGCGCTGGTGGCAATGACACCGAGCTGATAGCCTGCT TGAGGACAAGGCCCGCTCAGGACCTGGTGGACCACGAGTGGCACGTCCTGCCTCAAGAAAGTATCTTCCG ATTTTCCTTCGTGCCTGTGGTAGACGGGGACTTCCTCAGTGACACACCGGAGGCTCTCATCAATACTGGA GATTTTCAAGACCTGCAGGTGCTGGTGGGTGTGGTGAAGGACGAGGGCTCCTACTTTCTGGTTTACGGGG TCCCAGGCTTCAGCAAAGACAATGAATCTCTCATCAGCCGGGCCCAGTTCCTGGCTGGGGTGCGGATCGG TGTACCCCAAGCAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATTACACAGACTGGCTGCACCCTGAG GACCCTACTCACCTGAGAGATGCCATGAGTGCAGTGGTAGGCGACCACAACGTTGTGTGCCCTGTGGCCC AGCTGGCTGGGCGACTGGCTGCCCAAGGGGCCCGGGTCTATGCCTACATCTTTGAACACCGTGCCTCCAC ACTGACTTGGCCCCTCTGGATGGGGGTGCCCCATGGCTATGAAATCGAGTTCATCTTTGGGCTCCCCCTG GATCCCTCGCTGAACTACACCACGGAGGAGAGGATCTTTGCTCAGCGACTTATGAAATACTGGACCAATT TTGCCCGCACAGGGGACCCCAATGACCCTCGAGACTCCAAATCTCCACAGTGGCCACCGTACACCACTGC CGCGCAGCAATATGTGAGCCTGAACCTGAAGCCCTTAGAGGTGCGGCGGGGACTGCGCGCCCAGACCTGC GCCTTCTGGAATCGCTTTCTCCCCAAATTGCTCAGCGCCACCGATACTCTGGACGAGGCGGAGCGCCAGT GGAAGGCCGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTATAGCAA GCAGGAGCGCTGCTCAGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226310 representing NM_009599
Red=Cloning site Green=Tags(s) MRPPWYPLHTPSLAFPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAE PPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPA SPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLA LQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRAT LLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTG DFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPE DPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPL DPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTC AFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009599 |
| ORF Size | 1842 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009599.4, NP_033729.1 |
| RefSeq Size | 2184 bp |
| RefSeq ORF | 1845 bp |
| Locus ID | 11423 |
| UniProt ID | P21836 |
| MW | 68.6 kDa |
| Gene Summary | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219690 | Ache (untagged) - Mouse acetylcholinesterase (Ache), (10ug) |
CNY 6,864.00 |
|
| MG226310 | Ache (tGFP-tagged) - Mouse acetylcholinesterase (Ache), (10ug) |
CNY 6,650.00 |
|
| MR226310L3 | Lenti ORF clone of Ache (Myc-DDK-tagged) - Mouse acetylcholinesterase (Ache) |
CNY 7,980.00 |
|
| MR226310L4 | Lenti ORF clone of Ache (mGFP-tagged) - Mouse acetylcholinesterase (Ache) |
CNY 7,980.00 |
