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Clgn (NM_009904) Mouse Untagged Clone
CAT＃: MC219604
Clgn (untagged) - Mouse calmegin (Clgn), (10ug)
CN¥ 6,832.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4930459O04Rik; A2/; A2/6; AI528775; C; calne; Cln |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219604 representing NM_009904
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTTCCAGGGTGTTGGACTATGTTTGGGTCTCCTGTTCATCACGGTCAATGCGGACTTTATGGATG ATGGTGTTGAGGTGGAAGACTTCAGTGAAAATTCAGACGAGAGTAATATTAAAGATGAACCTTCCTCGGG GACTTTTAAGTATAAGACACCTCAACCTATAGGAGAAGTATATTTTACAGAAACATTTGATAGTGGAAAT CTGGCTGGGTGGGTCTTATCAAAAGCAAAGAAGGATGATATGGATTCAGAAATTGCTATATATGATGGAA GATGGGAAATAGAAGAACTGAAGGAAAACCAGGTGCCTGGCGACAGAGGGCTGGTTCTGAAGTCTAAAGC CAAGCACCACGCAATAGCCGCTGTCCTCGAAAAACCCTTTATTTTTGCCGATAAACCTTTGATAGTTCAA TATGAAGTAAATTTTCAAGATGGTATTGATTGTGGAGGTGCATACATTAAACTGCTAGCAGACACTGGTG ATTTGATTCTGGAAAACTTTTATGATAAAACATCCTATACCATCATGTTTGGACCAGATAAATGTGGAGA GGATTATAAACTTCATTTAATCTTCCGACACAAACATCCGAAAACTGGAGTTTTTGAAGAAAAGCATGCC AAGCCTCCAGATGTCGATCTGAAGGAGTTCTTTACAGACAGGAAGACTCACCTCTATACCCTTGTGATGA ATCCGGATGATACATTTGAAGTATTAATTGATCAGAAAGTTGTGAACCAAGGGACCTTGCTAGATGATGT GGTCCCGCCTATCAACCCACCCAGAGAAATTGACGACCCCAGTGATAAAAAGCCAGAGGAGTGGGATGAC AGAGCCAAGATCCCCGACCCTACCGCCGTCAGGCCAGAGGACTGGGATGAAAATGAACCTGCCCAAATAG AAGACTCAAGTGCTGTTAAACCTGATGGCTGGCTTGATGATGAACCAAAGTTTATCCCCAATCCTAAAGC TGAGAAACCTGAGGACTGGAGTGACGACATGGATGGAGAATGGGAGGCACCACATATTCCTAATCCAGCC TGTCAGATTGGATGTGGAGAATGGAAACCTCCCATGATCGATAACCCAAAATACAAAGGAATCTGGAGAC CACCAATGATCAATAATCCTAACTACCAGGGACTCTGGAGTCCTCAGAAGATTCCCAACCCAGATTACTT TGAGGATGACCACCCCTTCCTCCTGACTTCTTTCAGTGCGCTGGGCTTAGAGCTTTGGTCCATGACTCCT GACATCTACTTTGATAATTTTATCATCTGTTCAGAAAAGGAGGTGGCCGATCAGTGGGCTACAGATGGCT GGGAACTGAAGATAATGGTGGCAAATGCTAATGAGCCTGGTGTACTCCGACAGCTGGTGATAGCCGCAGA GGAGCGCCCCTGGCTCTGGCTCATGTACCTGGTGATGGCCGGCTTGCCCGTTGCATTAGTTGCTTCCTTT TGTTGGCCAAGGAAAGTAAAGAAAAAATATGAAGACACGGGACCTAAAAAAACTGAATTATGCAAACTGC AAAGCAAGGCCGCTCTGGAACAAGAGGCAGAGGAGGAGAAGGCCCCAGAGAAGCCAGAAGATGTGCAAGA AGAAAAGAAGCCAGGTGAAGCTGAGGTCGTCACCGTGGAAAAGGAAGTAATTGGTGAACCGGAAGAGAAG AGTAAGGAAGACAGAGAAACTCTAGAAGGTCAAGAGGAAGTGAGCAAGCTGAGTAAATCTGGATCAGAGG ATGAGATGAAGGACGCTGATGAGAGCCCAGGATCTGGAGATGCCCCACTGAAGTCATTGCGGAAGAGGAG GGTACGGAAGGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009904 |
| Insert Size | 1836 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009904.3, NP_034034.2 |
| RefSeq Size | 2307 bp |
| RefSeq ORF | 1836 bp |
| Locus ID | 12745 |
| UniProt ID | P52194 |
| Gene Summary | This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209394 | Clgn (tGFP-tagged) - Mouse calmegin (Clgn) |
CN¥ 6,650.00 |
|
| MR209394 | Clgn (Myc-DDK-tagged) - Mouse calmegin (Clgn) |
CN¥ 6,824.00 |
|
| MR209394L3 | Lenti ORF clone of Clgn (Myc-DDK-tagged) - Mouse calmegin (Clgn) |
CN¥ 7,980.00 |
|
| MR209394L4 | Lenti ORF clone of Clgn (mGFP-tagged) - Mouse calmegin (Clgn) |
CN¥ 7,980.00 |
