Gata6 (NM_010258) Mouse Untagged Clone
CAT#: MC219384
Gata6 (untagged) - Mouse GATA binding protein 6 (Gata6), (10ug)
CNY 6,592.00
Cited in 2 publications. |
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AA410133; GATA-6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NM_010258.3
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGACTGACGGCGGCTGGTGCCTGCCAAAGCGTTTCGGGGCTGCTGCTGCGGACGCCGGCGACT CCGGGCCCTTTCCAGCGCGGGAGCCCTCCTCGCCGCTTTCCCCCATCTCGTCTTCGTCCTCCTCCTGCTC CCGGGGCGGGGATCGCGGTCCCTGCGGCGCCAGCAACTGCAGGACGCCGCAGCTCGACGCCGAGGCGGTG GCGGGACCTCCGGGCCGCTCGCTCTTGCTCAGCCCCTACGCCTCGCATCCCTTCGCCGCTGCCCACGGAG CCGCGGCGCCCGGGGTCGCAGGCCCCGGGAGCGCCCTGTCGACTTGGGAGGACCTGTTGCTCTTCACTGA CCTCGATCAGGCCGCGACCGCCAGCAAGCTGTTGTGGTCCAGCCGGGGCGCCAAACTGAGCCCCTTCGCG GCCGAGCAGCCGGAGGAAATGTACCAGACCCTCGCCGCCCTGTCCAGCCAGGGGCCCGCCGCTTACGACG GCGCGCCCGGCGGCTTCGTGCACTCCGCAGCGGCGGCGGCCGCTGCCGCCGCGGCAGCCAGCTCCCCGGT CTACGTGCCCACCACGCGCGTGGGCTCCATGCTGTCCGGCCTGCCCTACCTTCAAGGGGCGGGCAGCGGG CCCAGCAATCACGCGGGCGGAGCGGGTGCCCACCCAGGCTGGTCCCAGGCCTCCGCCGACAGCCCCCCGT ATGGCGGGGGTGGCGCAGCCGGCGGCGGCGCGGCCGGACCTGGAGGTGCGGGATCGGCTACGGCCCACGC CTCTGCACGCTTTCCCTACTCGCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGAGACCCCGGGGGCTAC GTGGCTGCGGGCGGCACGGGCGCAGGCAGTGTGAGTGGAGGTGGCGGCAGCCTGGCGGCCATGGGTGGCC GGGAGCACCAGTACAGCTCGCTGTCCGCAGCTCGGCCGCTGAACGGAACGTACCACCACCACCATCACCA TCACCCGACCTACTCGCCCTACATGGCCGCACCGCTGACTCCTGCCTGGCCAGCAGGACCCTTCGAAACG CCGGTGCTCCACAGCTTACAGGGCCGCGCGGGAGCTCCACTCCCGGTGCCACGGGGCCCCAGCACAGACC TGTTGGAGGACCTGTCGGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCAGACGCCACTGTGGAGACG AGACGGCACCGGTCATTACCTGTGCAATGCATGCGGTCTCTACAGCAAGATGAATGGCCTCAGCAGGCCC CTCATCAAGCCACAGAAGCGCGTGCCTTCATCACGGCGGCTTGGACTGTCCTGTGCCAACTGTCACACCA CAACCACTACCTTATGGCGTAGAAATGCTGAGGGTGAGCCTGTGTGCAATGCTTGCGGGCTCTATATGAA ACTCCATGGGGTGCCTCGACCACTTGCTATGAAAAAAGAAGGAATTCAAACCAGGAAACGAAAACCTAAA AATATAAATAAGTCAAAAGCTTGCTCCGGTAACAGCAGTGGCTCTGTCCCTATGACTCCTACTTCCTCTT CTTCTAATTCAGATGACTGCACCAAAAATACTTCTCCTTCTACACAAGCGACCACCTCAGGGGTAGGGGC ATCAGTGATGTCTGCAGTGGGAGAAAACGCCAACCCCGAGAACAGTGACCTCAAGTATTCAGGTCAAGAC GGCCTCTACATAGGTGTCAGTCTGTCCTCCCCTGCCGAAGTCACATCCTCCGTGCGACAGGATTCTTGGT GTGCTCTGGCCCTGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_010258 |
Insert Size | 1770 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_010258.3, NP_034388.2 |
RefSeq Size | 3194 bp |
RefSeq ORF | 1770 bp |
Locus ID | 14465 |
UniProt ID | Q61169 |
Gene Summary | Transcriptional activator that regulates SEMA3C and PLXNA2 (PubMed:19666519). May regulate genes that protect epithelial cells from bacterial infection (By similarity). Involved in gene regulation specifically in the gastric epithelium (By similarity). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343).[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
A tissue-specific, Gata6-driven transcriptional program instructs remodeling of the mature arterial tree
,Losa, M;Latorre, V;Andrabi, M;Ladam, F;Sagerström, C;Novoa, A;Zarrineh, P;Bridoux, L;Hanley, NA;Mallo, M;Bobola, N;,
Elife
,PubMed ID 28952437
[GATA6]
|
Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells
,Kathy K Niakan, Nadine Schrode, Lily T Y Cho & Anna-Katerina Hadjantonakis,
Nature Protocols 8, 1028-1041 doi:10.1038/nprot.2013.049
,PubMed ID 23640167
[GATA6]
|
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Other Versions
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