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Gpc3 (NM_016697) Mouse Untagged Clone
CAT＃: MC219262
Gpc3 (untagged) - Mouse glypican 3 (Gpc3), (10ug)
CNY 4,320.00
CNY 7,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | OCI-5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219262 representing NM_016697
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGACCGTGCGCACCGCGTGCTTGCTGGTGGCGATGCTGCTAGGCTTGGGCTGCCTGGGACAGG CGCAGCCCCCGCCGCCTCCAGACGCCACCTGTCACCAGGTCCGTTCTTTCTTCCAGAGACTGCAGCCCGG ACTCAAATGGGTTCCAGAAACCCCTGTACCAGGATCAGATTTGCAAGTATGTCTCCCCAAGGGCCCAACA TGCTGCTCAAGAAAGATGGAAGAAAAATACCAACTAACAGCACGGCTGAACATGGAACAACTGCTCCAGT CTGCGAGTATGGAACTCAAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGT TGTTCGCCATGCCAAGAACTACACCAACGCCATGTTCAAGAATAACTACCCCAGCCTGACTCCACAAGCT TTTGAGTTTGTCGGTGAATTTTTCACAGATGTGTCTCTCTACATCTTGGGTTCTGATATCAACGTGGATG ATATGGTCAATGAATTGTTCGACAGCCTCTTTCCAGTCATCTACACCCAGATGATGAACCCAGGCCTGCC TGAGTCAGTCTTAGACATCAACGAGTGCCTCCGAGGAGCAAGACGTGACCTGAAAGTATTTGGCAGTTTC CCCAAGCTTATTATGACCCAGGTTTCCAAGTCACTGCAAGTCACTCGAATCTTCCTTCAAGCCCTGAATC TCGGAATTGAAGTCATCAACACTACCGACCACCTCAAGTTTAGTAAGGACTGTGGCCGTATGCTCACCCG AATGTGGTATTGCTCTTACTGCCAGGGACTGATGATGGTTAAGCCTTGCGGTGGTTATTGCAATGTGGTC ATGCAAGGCTGTATGGCTGGTGTGGTGGAGATCGACAAGTACTGGAGAGAATACATTCTGTCTCTTGAAG AGCTCGTGAATGGCATGTACAGAATCTACGACATGGAGAATGTGCTGCTCGGCCTCTTTTCTACCATCCA TGATTCCATCCAGTATGTGCAGAAGAACGGAGGCAAGCTGACCACCACCATTGGCAAGTTGTGTGCCCAC TCCCAGCAACGCCAATATAGATCTGCTTATTACCCTGAAGATCTGTTTATTGACAAGAAGATATTAAAAG TCGCTCATGTCGAACATGAAGAAACCTTATCCAGCCGAAGAAGGGAACTGATTCAGAAACTGAAGTCTTT CATCAACTTCTATAGCGCTTTGCCGGGCTACATCTGCAGCCATAGCCCCGTGGCCGAAAATGATACCCTG TGCTGGAACGGACAAGAACTTGTGGAGAGATACAGCCAGAAGGCGGCAAGGAACGGGATGAAGAATCAGT TTAACCTCCATGAGCTGAAAATGAAGGGCCCTGAGCCGGTGGTTAGCCAGATCATTGACAAACTGAAGCA CATTAACCAGCTCCTGAGAACCATGTCTGTGCCCAAGGGTAAAGTTCTGGATAAAAGCCTGGATGAAGAA GGACTTGAAAGTGGAGACTGCGGTGATGATGAAGATGAATGCATTGGAAGCTCTGGTGACGGGATGGTGA AAGTGAAGAATCAACTGCGCTTCCTTGCAGAACTGGCCTATGATCTGGATGTGGACGATGCTCCGGGGAA CAAGCAGCATGGAAATCAGAAGGACAACGAGATCACCACCTCTCACAGCGTGGGGAACATGCCGTCCCCA CTGAAGATCCTCATCAGTGTGGCCATCTATGTGGCGTGCTTTTTTTTCCTGGTGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016697 |
| Insert Size | 1740 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC036126, AAH36126 |
| RefSeq Size | 2312 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 14734 |
| UniProt ID | Q8CFZ4 |
| Gene Summary | Cell surface proteoglycan that bears heparan sulfate (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed:18477453, PubMed:23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed:18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed:15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed:10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed:11180950). Required for coronary vascular development (PubMed:19733558). Plays a role in regulating cell movements during gastrulation (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209076 | Gpc3 (tGFP-tagged) - Mouse glypican 3 (Gpc3) |
CNY 5,040.00 |
|
| MR209076 | Gpc3 (Myc-DDK-tagged) - Mouse glypican 3 (Gpc3) |
CNY 4,320.00 |
|
| MR209076L3 | Lenti ORF clone of Gpc3 (Myc-DDK-tagged) - Mouse glypican 3 (Gpc3) |
CNY 6,720.00 |
|
| MR209076L4 | Lenti ORF clone of Gpc3 (mGFP-tagged) - Mouse glypican 3 (Gpc3) |
CNY 6,460.00 |
