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Gpc3 (NM_016697) Mouse Tagged ORF Clone
CAT＃: MG209076
- TrueORF®
Gpc3 (tGFP-tagged) - Mouse glypican 3 (Gpc3)
ORF Plasmid: DDK
"NM_016697" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | OCI-5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209076 representing NM_016697
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGACCGTGCGCACCGCGTGCTTGCTGGTGGCGATGCTGCTAGGCTTGGGCTGCCTGGGACAGG CGCAGCCCCCGCCGCCTCCAGACGCCACCTGTCACCAGGTCCGTTCTTTCTTCCAGAGACTGCAGCCCGG ACTCAAATGGGTTCCAGAAACCCCTGTACCAGGATCAGATTTGCAAGTATGTCTCCCCAAGGGCCCAACA TGCTGCTCAAGAAAGATGGAAGAAAAATACCAACTAACAGCACGGCTGAACATGGAACAACTGCTCCAGT CTGCGAGTATGGAACTCAAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGT TGTTCGCCATGCCAAGAACTACACCAACGCCATGTTCAAGAATAACTACCCCAGCCTGACTCCACAAGCT TTTGAGTTTGTCGGTGAATTTTTCACAGATGTGTCTCTCTACATCTTGGGTTCTGATATCAACGTGGATG ATATGGTCAATGAATTGTTCGACAGCCTCTTTCCAGTCATCTACACCCAGATGATGAACCCAGGCCTGCC TGAGTCAGTCTTAGACATCAACGAGTGCCTCCGAGGAGCAAGACGTGACCTGAAAGTATTTGGCAGTTTC CCCAAGCTTATTATGACCCAGGTTTCCAAGTCACTGCAAGTCACTCGAATCTTCCTTCAAGCCCTGAATC TCGGAATTGAAGTCATCAACACTACCGACCACCTCAAGTTTAGTAAGGACTGTGGCCGTATGCTCACCCG AATGTGGTATTGCTCTTACTGCCAGGGACTGATGATGGTTAAGCCTTGCGGTGGTTATTGCAATGTGGTC ATGCAAGGCTGTATGGCTGGTGTGGTGGAGATCGACAAGTACTGGAGAGAATACATTCTGTCTCTTGAAG AGCTCGTGAATGGCATGTACAGAATCTACGACATGGAGAATGTGCTGCTCGGCCTCTTTTCTACCATCCA TGATTCCATCCAGTATGTGCAGAAGAACGGAGGCAAGCTGACCACCACCATTGGCAAGTTGTGTGCCCAC TCCCAGCAACGCCAATATAGATCTGCTTATTACCCTGAAGATCTGTTTATTGACAAGAAGATATTAAAAG TCGCTCATGTCGAACATGAAGAAACCTTATCCAGCCGAAGAAGGGAACTGATTCAGAAACTGAAGTCTTT CATCAACTTCTATAGCGCTTTGCCGGGCTACATCTGCAGCCATAGCCCCGTGGCCGAAAATGATACCCTG TGCTGGAACGGACAAGAACTTGTGGAGAGATACAGCCAGAAGGCGGCAAGGAACGGGATGAAGAATCAGT TTAACCTCCATGAGCTGAAAATGAAGGGCCCTGAGCCGGTGGTTAGCCAGATCATTGACAAACTGAAGCA CATTAACCAGCTCCTGAGAACCATGTCTGTGCCCAAGGGTAAAGTTCTGGATAAAAGCCTGGATGAAGAA GGACTTGAAAGTGGAGACTGCGGTGATGATGAAGATGAATGCATTGGAAGCTCTGGTGACGGGATGGTGA AAGTGAAGAATCAACTGCGCTTCCTTGCAGAACTGGCCTATGATCTGGATGTGGACGATGCTCCGGGGAA CAAGCAGCATGGAAATCAGAAGGACAACGAGATCACCACCTCTCACAGCGTGGGGAACATGCCGTCCCCA CTGAAGATCCTCATCAGTGTGGCCATCTATGTGGCGTGCTTTTTTTTCCTGGTGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209076 representing NM_016697
Red=Cloning site Green=Tags(s) MAGTVRTACLLVAMLLGLGCLGQAQPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPT CCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQA FEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQMMNPGLPESVLDINECLRGARRDLKVFGSF PKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVV MQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNGGKLTTTIGKLCAH SQQRQYRSAYYPEDLFIDKKILKVAHVEHEETLSSRRRELIQKLKSFINFYSALPGYICSHSPVAENDTL CWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGKVLDKSLDEE GLESGDCGDDEDECIGSSGDGMVKVKNQLRFLAELAYDLDVDDAPGNKQHGNQKDNEITTSHSVGNMPSP LKILISVAIYVACFFFLVH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016697 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016697.3 |
| RefSeq Size | 2312 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 14734 |
| UniProt ID | Q8CFZ4 |
| Gene Summary | Cell surface proteoglycan that bears heparan sulfate (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed:18477453, PubMed:23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed:18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed:15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed:10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed:11180950). Required for coronary vascular development (PubMed:19733558). Plays a role in regulating cell movements during gastrulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219262 | Gpc3 (untagged) - Mouse glypican 3 (Gpc3), (10ug) |
CNY 4,320.00 |
|
| MR209076 | Gpc3 (Myc-DDK-tagged) - Mouse glypican 3 (Gpc3) |
CNY 4,320.00 |
|
| MR209076L3 | Lenti ORF clone of Gpc3 (Myc-DDK-tagged) - Mouse glypican 3 (Gpc3) |
CNY 6,460.00 |
|
| MR209076L4 | Lenti ORF clone of Gpc3 (mGFP-tagged) - Mouse glypican 3 (Gpc3) |
CNY 6,460.00 |
