Bag3 (NM_013863) Mouse Untagged Clone

CAT#: MC219180

Bag3 (untagged) - Mouse BCL2-associated athanogene 3 (Bag3), (10ug)



  "NM_013863" in other vectors (6)

CNY 7,320.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA407278; Bis; mg638
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219180 representing NM_013863
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCCGCCACCCAATCGCCCATGATGCAGATGGCGTCCGGCAACGGAGCCAGCGACCGAGACCCCC
TGCCCCCGGGCTGGGAGATCAAAATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCG
CACCACGACGTGGAACGACCCGCGTGTGCCCCCCGAGGGCCCTAAGGACACTGCATCTTCAGCCAATGGC
CCTTCCCGCGATGGCTCCCGCCTGCTGCCCATTAGGGAAGGCCACCCCATATACCCCCAGCTCCGACCAG
GCTACATTCCCATCCCCGTCCTCCATGAAGGCTCCGAGAACCGGCAGCCCCACCTTTTCCATGCCTACTC
CCAGCCTGGGGTGCAGCGATTTCGTACCGAGGCTGCAGCGGCCACTCCGCAGAGGTCCCAGTCTCCTTTA
CGTGGCGGCATGACAGAAGCCGCCCAGACAGATAAACAGTGTGGACAGATGCCGGCAACTGCAACAACTG
CAGCAGCTCAGCCCCCAACTGCTCATGGACCTGAGCGATCTCAGTCTCCAGCTGCCTCTGACTGCTCATC
CTCTTCCTCCTCGGCCAGCCTGCCCTCCTCCGGCAGGAGCAGTCTGGGTAGCCATCAGCTCCCCAGGGGC
TACATCCCCATCCCCGTGATACATGAGCAGAATATCACCCGGCCAGCAGCCCAGCCCTCCTTCCACCAAG
CCCAGAAGACCCACTACCCAGCTCAGCAGGGTGAATACCAGCCCCAGCAGCCTGTATACCACAAGATCCA
GGGCGATGACTGGGAGCCCCGGCCGCTTCGAGCCGCTTCTCCATTCAGGTCACCCGTCAGAGGTGCTTCC
AGCCGGGAAGGCTCGCCCGCCAGAAGCGGCACACCGGTTCACTGCCCATCTCCCATCCGTGTGCACACAG
TGGTTGACAGGCCTCAGCCCATGACCCATCGAGAGCCTCCACCTGTTACCCAACCCGAAAACAAACCAGA
AAGTAAGCCAGGCCCAGCCGGACCAGACCTCCCTCCTGGACACATCCCCATCCAAGTGATCCGAAGGGAG
GCAGACTCTAAGCCTGTTTCCCAGAAGTCACCTCCTCCTGCTGAAAAGGTAGAAGTAAAGGTCTCCTCTG
CTCCAATTCCTTGTCCTTCTCCCAGCCCCGCTCCTTCGGCTGTCCCTTCTCCCCCCAAGAATGTGGCTGC
AGAACAGAAGGCGGCCCCCAGCCCTGCCCCTGCAGAACCTGCAGCCCCCAAATCGGGAGAAGCCGAAACA
CCCCCTAAGCATCCAGGTGTGCTGAAAGTGGAAGCCATCCTGGAGAAGGTACAAGGGCTGGAGCAGGCTG
TAGACAGCTTTGAAGGCAAGAAGACTGATAAAAAATACCTGATGATCGAAGAGTATTTGACCAAAGAGCT
GCTGGCCCTGGATTCCGTAGACCCTGAAGGACGAGCTGATGTGCGGCAGGCCAGGAGAGATGGCGTCAGG
AAGGTTCAGACCATCTTGGAAAAACTTGAGCAGAAAGCAATTGATGTCCCAGGTCAAGTACAAGTCTATG
AACTCCAGCCCAGCAACCTTGAGGCCGAGCAGCCACTCCAGGAAATCATGGGTGCCGTGGTAGCCGACAA
GGATAAGAAAGGTCCTGAAAACAAAGATCCACAAACTGAAAGCCAACAGCTAGAAGCCAAAGCAGCCACA
CCTCCAAATCCCAGCAACCCAGCAGACTCAGCTGGCAACCTGGTGGCTCCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_013863
Insert Size 1734 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013863.5, NP_038891.4
RefSeq Size 2607 bp
RefSeq ORF 1734 bp
Locus ID 29810
UniProt ID Q9JLV1
Gene Summary Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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