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Bag3 (NM_013863) Mouse Tagged ORF Clone
CAT＃: MR226055
- TrueORF®
Bag3 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 3 (Bag3)
ORF Plasmid: tGFP
"NM_013863" in other vectors (6)
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CNY 6,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA407278; Bis; mg638 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226055 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCCGCCACCCAATCGCCCATGATGCAGATGGCGTCCGGCAACGGAGCCAGCGACCGAGACCCCC TGCCCCCGGGCTGGGAGATCAAAATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCG CACCACGACGTGGAACGACCCGCGTGTGCCCCCCGAGGGCCCTAAGGACACTGCATCTTCAGCCAACGGC CCTTCCCGCAATGGCTCCCGCCTGCTGCCCATTAGGGAAGGCCACCCCATATACCCCCAGCTCCGACCAG GCTACATTCCCATCCCCGTCCTCCATGAAGGCTCCGAGAACCGGCAGCCCCACCTTTTCCATGCCTACTC CCAGCCTGGGGTGCAGCGATTTCGTACCGAGGCTGCAGCGGCCACTCCGCAGAGGTCCCAGTCTCCTTTA CGTGGCGGCATGACAGAAGCCGCCCAGACAGATAAACAGTGTGGACAGATGCCGGCAACTGCAACAACTG CAGCAGCTCAGCCCCCAACTGCTCATGGACCTGAGCGATCTCAGTCTCCAGCTGCCTCTGACTGCTCATC CTCTTCCTCCTCGGCCAGCCTGCCCTCCTCCGGCAGGAGCAGTCTGGGTAGCCATCAGCTCCCCAGGGGC TACATCCCCATCCCCGTGATACATGAGCAGAATATCACCCGGCCAGCAGCCCAGCCCTCCTTCCACCAAG CCCAGAAGACCCACTACCCAGCTCAGCAGGGTGAATACCAGCCCCAGCAGCCTGTATACCACAAGATCCA GGGCGATGACTGGGAGCCCCGGCCGCTTCGAGCCGCTTCTCCATTCAGGTCACCCGTCAGAGGTGCTTCC AGCCGGGAAGGCTCGCCCGCCAGAAGCGGCACACCGGTTCACTGCCCATCTCCCATCCGTGTGCACACAG TGGTTGACAGGCCTCAGCCCATGACCCATCGAGAGCCTCCACCTGTTACCCAACCCGAAAACAAACCAGA AAGTAAGCCAGGCCCAGCCGGACCAGACCTCCCTCCTGGACACATCCCCATCCAAGTGATCCGAAGGGAG GCAGACTCTAAGCCTGTTTCCCAGAAGTCACCTCCTCCTGCTGAAAAGGTAGAAGTAAAGGTCTCCTCTG CTCCAATTCCTTGTCCTTCTCCCAGCCCCGCTCCTTCGGCTGTCCCTTCTCCCCCCAAGAATGTGGCTGC AGAACAGAAGGCGGCCCCCAGCCCTGCCCCTGCAGAACCTGCAGCCCCCAAATCGGGAGAAGCCGAAACA CCCCCTAAGCATCCAGGTGTGCTGAAAGTGGAAGCCATCCTGGAGAAGGTACAAGGGCTGGAGCAGGCTG TAGACAGCTTTGAAGGCAAGAAGACTGATAAAAAATACCTGATGATCGAAGAGTATTTGACCAAAGAGCT GCTGGCCCTGGATTCCGTAGACCCTGAAGGACGAGCTGATGTGCGGCAGGCCAGGAGAGATGGCGTCAGG AAGGTTCAGACCATCTTGGAAAAACTTGAGCAGAAAGCAATTGATGTCCCAGGTCAAGTACAAGTCTATG AACTCCAGCCCAGCAACCTTGAGGCCGAGCAGCCACTCCAGGAAATCATGGGTGCCGTGGTAGCCGACAA GGATAAGAAAGGTCCTGAAAACAAAGATCCACAAACTGAAAGCCAACAGCTAGAAGCCAAAGCAGCCACG CCTCCAAATCCCAGCAACCCAGCAGACTCAGCTGGCAACCTGGTGGCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226055 protein sequence
Red=Cloning site Green=Tags(s) MSAATQSPMMQMASGNGASDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPPEGPKDTASSANG PSRNGSRLLPIREGHPIYPQLRPGYIPIPVLHEGSENRQPHLFHAYSQPGVQRFRTEAAAATPQRSQSPL RGGMTEAAQTDKQCGQMPATATTAAAQPPTAHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRG YIPIPVIHEQNITRPAAQPSFHQAQKTHYPAQQGEYQPQQPVYHKIQGDDWEPRPLRAASPFRSPVRGAS SREGSPARSGTPVHCPSPIRVHTVVDRPQPMTHREPPPVTQPENKPESKPGPAGPDLPPGHIPIQVIRRE ADSKPVSQKSPPPAEKVEVKVSSAPIPCPSPSPAPSAVPSPPKNVAAEQKAAPSPAPAEPAAPKSGEAET PPKHPGVLKVEAILEKVQGLEQAVDSFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVR KVQTILEKLEQKAIDVPGQVQVYELQPSNLEAEQPLQEIMGAVVADKDKKGPENKDPQTESQQLEAKAAT PPNPSNPADSAGNLVAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013863 |
| ORF Size | 1734 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013863.1, NM_013863.2, NM_013863.3, NM_013863.4, NM_013863.5, NP_038891.4 |
| RefSeq Size | 2607 bp |
| RefSeq ORF | 1734 bp |
| Locus ID | 29810 |
| UniProt ID | Q9JLV1 |
| MW | 61.9 kDa |
| Gene Summary | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219180 | Bag3 (untagged) - Mouse BCL2-associated athanogene 3 (Bag3), (10ug) |
CNY 7,320.00 |
|
| MG226055 | Bag3 (tGFP-tagged) - Mouse BCL2-associated athanogene 3 (Bag3), (10ug) |
CNY 8,056.00 |
|
| MR226055L1 | Lenti ORF clone of Bag3 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 3 (Bag3) |
CNY 7,700.00 |
|
| MR226055L2 | Lenti ORF clone of Bag3 (mGFP-tagged) - Mouse BCL2-associated athanogene 3 (Bag3) |
CNY 8,856.00 |
|
| MR226055L3 | Lenti ORF clone of Bag3 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 3 (Bag3) |
CNY 7,700.00 |
|
| MR226055L4 | Lenti ORF clone of Bag3 (mGFP-tagged) - Mouse BCL2-associated athanogene 3 (Bag3) |
CNY 7,700.00 |
