Selenon (NM_029100) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Synonyms	1110019I12Rik; AI414492; Se; SelN; Sepn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC218987 representing NM_029100 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGGCCAGGCCCGCAGCGCGCCGCCCGCACAGCCCGGACCCCGGCGCTCAGCCCGCGCCTCCAC GCCGCCGCGCCCGCGCCCTGGCGCTGCTCGGAGCCCTGCTGGCGGCCGCCGCCGCTGTCGCCGCTGCCCG GGCCTGCGCGCTCCTCGCCGACGCCCAGGCGGCCGCGCGGCAGGAATCGGCATTAAAGGTCCTGGGGACA GATGGCCTCTTTCTCTTTTCCTCGCTGGACACTGACCAGGATATGTACATCAGTCCGGAGGAATTCAAAC CCATTGCGGAGAAACTGACAGGGTCAGTTCCTGTGGCCAACTACGAGGAGGAGGAACTGCCCCATGACCC CAGCGAGGAGACTCTTACCATAGAAGCCCGATTCCAGCCTCTGCTCATGGAGACCATGACCAAAAGCAAA GATGGCTTCCTAGGGGTCTCCCGCCTCGCTCTGTCCGGCCTCCGCAACTGGACCACAGCAGCCTCGCCGA GTGCAGCGTTTGCTGCCCGCCACTTCCGGCCCTTCCTGCCCCCTCCAGGTCAGGAGCTGGGCCAGCCCTG GTGGATTATCCCCGGGGAGCTGAGCGTCTTCACGGGCTATTTGTCCAACAACCGCTTCTACCCACCACCG CCCAAGGGCAAGGAGGTCATCATCCATCGCCTGTTAAGCATGTTCCATCCACGCCCCTTCGTGAAGACCC GCTTTGCCCCTCAGGGCACCGTGGCCTGTCTGACTGCCATCAGCGATTCCTACTACACCGTGATGTTCCG GATCCACGCGGAGTTTCAGCTCAGCGAGCCTCCTGACTTCCCCTTCTGGTTCTCGCCCGGCCAGTTCACC GGCCATATCATCTTATCCAAAGATGCTACACACATCCGTGACTTCAGGCTGTTTGTGCCCAATCACAGGT CCCTGAATGTGGACATGGAGTGGCTGTATGGGGCCAGTGAGACCAGCAACATGGAGGTGGACATTGGCTA CGTCCCCCAGATGGAGCTGGAGGCTGTGGGCCCCTCGGTGCCCTCTGTGATCCTAGATGAGGACGGCAAC ATGATTGACAGCCGCCTGCCCTCAGGAGAACCCCTCCAGTTTGTGTTTGAGGAGATCAAGTGGCATCAGG AGCTGAGCTGGGAAGAGGCTGCCCGGCGCCTGGAGGTGGCCATGTATCCCTTCAAGAAGGTCAACTACCT GCCGTTCACGGAGGCCTTTGACAGAGCCAGAGCTGAGAAGAAACTTGTTCATTCCATCTTGCTGTGGGGG GCCCTGGACGACCAGTCCTGCTGAGGTTCCGGGCGGACTCTCCGGGAGACTGTCCTGGAAAGCCCGCCCA TCCTCACTCTCCTCAATGAGAGCTTCATCAGTACCTGGTCCCTGGTAAAGGAGCTAGAAGACCTGCAGAC CCAGCAGGAGAACCCACTCCACAGGCAGCTGGCAGGCCTGCACTTGGAGAAGTACAGCTTTCCTGTAGAG ATGATGATCTGTCTGCCCAACGGCACTGTGGTCCACCACATCAATGCCAACTACTTCCTTGACATCACCT CCATGAAGCCTGAAGACATGGAGAATAATAACGTCTTCAGCTTCTCATCCAGCTTTGAGGACCCGTCCAC AGCTACCTACATGCAGTTCCTGAGGGAAGGACTCCGGCGGGGCCTGCCCCTCCTCCAGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029100
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029100.2, NP_083376.2
RefSeq Size	3461 bp
RefSeq ORF	1674 bp
Locus ID	74777
UniProt ID	D3Z2R5
Gene Summary	This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in the orthologous gene in human are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. Knockout mice deleted for this gene exhibit abnormal lung development. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. [provided by RefSeq, Dec 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.