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Arih1 (NM_019927) Mouse Untagged Clone
CAT＃: MC218921
Arih1 (untagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1), (10ug)
CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ari; Ari1; AU021774; Hari; Hhari; Ubch7bp; Uip77 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218921 representing NM_019927
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGACGAGGGCTACAACTACGAGTTCGACGAGGACGAGGAGTGCAGCGAGGAGGACAGCGGCG CCGAGGAGGAGGAGGACGACGACGAGGACGAGCCGGACGATGATAACCTGGATCTGGGCGAGGTGGAGCT GGTGGAGCCCGGGCTGGGCGTCGGCGGGGAGCGAGACGGACTGCTGTGCGGGGAGACTGGCGGTGGCGGC GGCAGCGCTCTGGGTCCGGGAGGCGGCGGCGGCGGTGGCGGTGGCGGCGGCGGCCCGGGACACGAGCAGG AGGAGGATTACCGCTACGAGGTGCTCACGGCCGAACAGATCCTGCAGCACATGGTGGAATGTATCCGGGA GGTCAACGAGGTCATCCAGAATCCAGCAACTATCACGAGAATACTCCTTAGCCACTTCAATTGGGATAAA GAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGCTGAGTGTCATGTAATTAATC CAAGTAAAAAGTCTCGAACTCGCCAGATGAATACAAGGTCATCGGCACAGGATATGCCGTGTCAGATCTG CTACTTGAACTACCCTAACTCGTATTTTACTGGCCTTGAATGTGGACATAAGTTTTGTATGCAGTGCTGG AGTGAATATTTAACTACAAAAATAATGGAGGAAGGCATGGGGCAGACTATTTCGTGTCCTGCGCATGGTT GTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCCAAAGTTAAGTTAAAATATCA ACATTTAATAACAAATAGCTTTGTAGAGTGCAACCGACTGTTAAAGTGGTGTCCTGCCCCAGATTGCCAC CATGTTGTTAAAGTCCAGTATCCTGATGCAAAACCAGTTCGCTGCAAATGTGGCCGCCAGTTTTGCTTTA ACTGTGGTGAAAATTGGCATGATCCAGTTAAATGTAAGTGGTTGAAGAAGTGGATTAAAAAGTGTGATGA TGACAGTGAAACTTCTAATTGGATTGCAGCTAACACAAAGGAATGTCCCAAATGCCATGTCACAATTGAG AAGGATGGCGGTTGTAATCACATGGTCTGTCGTAACCAGAACTGTAAAGCAGAATTTTGCTGGGTGTGTC TTGGCCCTTGGGAACCGCATGGATCTGCCTGGTACAACTGTAACCGCTATAATGAGGATGATGCAAAGGC AGCAAGAGATGCCCAAGAGCGATCTAGGGCGGCCCTGCAGAGATACCTGTTCTACTGTAATCGCTATATG AACCACATGCAGAGTCTACGCTTCGAGCATAAGCTGTATGCTCAGGTGAAACAGAAGATGGAGGAGATGC AGCAGCACAACATGTCCTGGATCGAGGTGCAGTTCCTGAAGAAAGCAGTTGATGTCCTCTGCCAGTGTCG TGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATAACCAGTCCATTATCTTTGAG AATAATCAAGCAGATCTAGAAAATGCCACAGAGGTGCTTTCGGGCTACCTTGAACGAGATATTTCCCAAG ATTCTCTGCAAGATATAAAGCAGAAAGTCCAAGATAAGTACAGATACTGTGAGAGTCGACGAAGGGTTTT GTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTGAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019927 |
| Insert Size | 1668 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019927.2, NP_064311.2 |
| RefSeq Size | 6435 bp |
| RefSeq ORF | 1668 bp |
| Locus ID | 23806 |
| UniProt ID | Q9Z1K5 |
| Gene Summary | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207099 | Arih1 (tGFP-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1) |
CNY 5,420.00 |
|
| MR207099 | Arih1 (Myc-DDK-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1) |
CNY 3,656.00 |
|
| MR207099L3 | Lenti ORF clone of Arih1 (Myc-DDK-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1) |
CNY 6,840.00 |
|
| MR207099L4 | Lenti ORF clone of Arih1 (mGFP-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1) |
CNY 6,840.00 |
