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Pgk1 (BC083355) Mouse Untagged Clone
CAT＃: MC218218
Pgk1 (untagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Pgk-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC083355
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG ACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACAACCAAAGGATCAAGGCTGCTGTTCCAAGCAT CAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGCCACCTGGGCCGGCCTGATGGTGTT CCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCTGAACTCAAATCTCTGCTGGGCAAGGATGTTC TGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT CCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGAAACAAGGTT AAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCACTGTCCAAACTAGGAGATGTCTATGTCAATG ATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT ATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA TGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT GTATGATGAAGAAGGAGCCAAGATTGTCAAAGATCTCATGTCCAAAGCTGAGAAAAATGGTGTGAAGATT ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCCAAGACTGGCCAAGCTACTGTGG CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGCAAGAAATATGCCGAGGC TGTGGGTCGAGCTAAGCAGATTGTTTGGAATGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG GGAACCAAGTCACTCATGGATGAGGTGGTGAAAGCCACTTCTAGGGGTTGCATCACTATCATAGGTGGTG GAGACACTGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG TGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC083355 |
| Insert Size | 1254 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC083355, AAH83355 |
| RefSeq Size | 1727 bp |
| RefSeq ORF | 1253 bp |
| Locus ID | 18655 |
| Gene Summary | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206611 | Pgk1 (tGFP-tagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) |
CNY 4,560.00 |
|
| MR206611 | Pgk1 (Myc-DDK-tagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) |
CNY 3,656.00 |
|
| MR206611L3 | Lenti ORF clone of Pgk1 (Myc-DDK-tagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) |
CNY 6,080.00 |
|
| MR206611L4 | Lenti ORF clone of Pgk1 (mGFP-tagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087) |
CNY 6,080.00 |
