Pgk1 (BC083355) Mouse Untagged Clone

CAT#: MC218218

Pgk1 (untagged) - Mouse phosphoglycerate kinase 1 (cDNA clone MGC:102158 IMAGE:6828087), (10ug)



  "BC083355" in other vectors (4)

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Pgk-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC083355
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG
ACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACAACCAAAGGATCAAGGCTGCTGTTCCAAGCAT
CAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGCCACCTGGGCCGGCCTGATGGTGTT
CCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCTGAACTCAAATCTCTGCTGGGCAAGGATGTTC
TGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT
CCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGAAACAAGGTT
AAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCACTGTCCAAACTAGGAGATGTCTATGTCAATG
ATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG
ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT
ATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA
TGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT
GTATGATGAAGAAGGAGCCAAGATTGTCAAAGATCTCATGTCCAAAGCTGAGAAAAATGGTGTGAAGATT
ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCCAAGACTGGCCAAGCTACTGTGG
CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGCAAGAAATATGCCGAGGC
TGTGGGTCGAGCTAAGCAGATTGTTTGGAATGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG
GGAACCAAGTCACTCATGGATGAGGTGGTGAAAGCCACTTCTAGGGGTTGCATCACTATCATAGGTGGTG
GAGACACTGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG
TGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC083355
Insert Size 1254 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC083355, AAH83355
RefSeq Size 1727 bp
RefSeq ORF 1253 bp
Locus ID 18655
Gene Summary The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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