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Smo (BC048091) Mouse Untagged Clone
CAT＃: MC218049
Smo (untagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D13Mgi8, Smoh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC048091
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCTGGCCGCCCCGTGCGTGGGCCCGAGCTGGCGCCCCGGAGGCTGCTGCAGTTGCTGCTGCTGG TACTGCTGGGGGGCCCGGGCCGGGGGGCGGCCTTGAGCGGGAACGTGACCGGGCCTGGGCCTCACAGCGC CAGCGGGAGCTCGAGGAGGAACGTGCCGGTGACCAGCCCTCCGCCGCCGCTGCTGAGCCACTGCGGCCGG GCCGCCCACTGCGAGCCTTTGCGCTACAACGTGTGCCTGGGCTCGGCGCTGCCCTACGGAGCCACCACCA CGCTGCTGGCTGGGGACTCGGACTCGCAGGAGGAAGCGCACGGCAAGCTCGTGCTCTGGTCCGGCCTCCG GAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGTGCTGTCTACATGCCCAAGTGTGAGAAT GACCGAGTGGAGTTGCCCAGCCGTACCCTCTGCCAGGCCACCCGAGGCCCCTGTGCCATTGTGGAGCGGG AGCGAGGGTGGCCTGACTTTCTGCGTTGCACACCGGACCACTTCCCTGAAGGCTGCCCAAACGAGGTACA AAACATCAAGTTCAACAGCTCAGGCCAATGTGAAGCACCCTTGGTGCGAACAGACAACCCCAAGAGCTGG TATGAGGACGTGGAGGGCTGTGGGATTCAGTGTCAGAACCCGCTGTTCACCGAGGCCGAGCACCAGGACA TGCACAGCTACATCGCAGCCTTCGGGGCGGTCACTGGTCTCTGCACGCTCTTCACACTGGCCACCTTTGT GGCTGACTGGCGGAACTCCAATCGCTACCCTGCGGTTATTCTCTTCTATGTCAATGCGTGTTTCTTCGTG GGCAGCATTGGCTGGCTGGCCCAGTTCATGGATGGTGCCCGCCGAGAGATTGTTTGCCGAGCAGATGGCA CCATGAGATTTGGGGAGCCCACCTCCAGTGAGACCCTGTCCTGTGTCATCATCTTTGTCATTGTGTACTA TGCCTTGATGGCTGGAGTAGTCTGGTTCGTGGTCCTCACCTATGCCTGGCACACCTCCTTCAAAGCCCTG GGCACCACCTACCAGCCTCTCTCGGGCAAGACATCCTATTTCCACCTGCTCACGTGGTCACTCCCCTTTG TCCTCACGGTGGCAATCCTGGCTGTGGCTCAGGTAGATGGAGACTCCGTGAGTGGCATCTGTTTTGTAGG CTACAAGAACTATCGGTACCGTGCTGGCTTTGTCCTGGCCCCAATTGGCCTGGTGCTTATTGTGGGAGGC TACTTCCTCATCACAGGGGTCATGACTCTGTTCTCCATCAAGAGCAACCACCCCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC048091 |
| Insert Size | 1320 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048091, AAH48091 |
| RefSeq Size | 1747 bp |
| RefSeq ORF | 1319 bp |
| Locus ID | 319757 |
| Gene Summary | G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO) (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206991 | Smo (tGFP-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) |
CNY 2,760.00 |
|
| MR206991 | Smo (Myc-DDK-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) |
CNY 3,656.00 |
|
| MR206991L3 | Lenti ORF clone of Smo (Myc-DDK-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) |
CNY 4,370.00 |
|
| MR206991L4 | Lenti ORF clone of Smo (mGFP-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) |
CNY 4,370.00 |
