Phgdh (NM_016966) Mouse Untagged Clone
CAT#: MC217652
Phgdh (untagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh), (10ug)
CNY 6,840.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 3-PGDH; 3PGDH; 4930479N23; A10; PGAD; PGD; PGDH; SERA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC217652 representing NM_016966
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTCGCAAATCTGCGCAAAGTACTCATCAGTGACAGCCTGGACCCCTGCTGCCGGAAGATCCTGC AAGATGGAGGGCTGCAGGTGGTGGAGAAGCAGAACTTGAGCAAGGAGGAGCTGATAGCTGAACTCCAGGA CTGTGAAGGCCTCATTGTCCGGTCTGCTACTAAGGTCACTGCTGATGTCATCAATGCAGCAGAGAAGCTC CAGGTGGTGGGCAGGGCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCTGCCACGAGGAAGGGCATCC TAGTCATGAACACCCCCAACGGGAACAGCCTCAGTGCTGCGGAGCTCACCTGTGGGATGATCATGTGCCT GGCCAGGCAGATTCCCCAGGCAACAGCTTCGATGAAAGATGGCAAATGGGACCGGAAGAAGTTCATGGGG ACAGAACTGAACGGGAAGACACTGGGAATTCTTGGCCTCGGCAGAATTGGAAGAGAGGTGGCCACCCGAA TGCAATCCTTTGGAATGAAGACTGTAGGCTATGACCCCATCATCTCTCCTGAAGTCGCCGCCTCCTTTGG TGTTCAGCAGCTGCCGCTGGAGGAGATCTGGCCTCTCTGTGACTTTATAACTGTCCATACCCCACTCCTG CCCTCTACCACAGGCTTGCTGAATGACAGCACCTTTGCTCAGTGCAAGAAAGGTGTGCGAGTAGTGAACT GTGCTCGAGGAGGCATTGTGGATGAAGGTGCCCTGCTGCGTGCCCTGCAGTCTGGTCAGTGTGCTGGGGC TGCACTGGATGTGTTTACAGAAGAGCCACCACGGGACCGGGCCTTAGTGGACCACGAGAATGTCATCAGC TGTCCCCACCTGGGTGCCAGCACCAAGGAAGCCCAGAGCCGCTGTGGGGAGGAAATCGCAGTCCAGTTTG TGGACATGGTGAAGGGGAAATCTCTAACAGGCGTTGTGAACGCCCAGGCCCTCACCAGTGCCTTCTCTCC ACACACCAAGCCTTGGATTGGTCTGGCAGAAGCAATGGGCACGCTGATGCACGCCTGGGCTGGCTCCCCT AAAGGGACCATCCAGGTGGTTACACAAGGAACATCTCTGAAGAATGCTGGGACCTGCCTGAGCCCTGCAG TCATTGTCGGCCTTCTGAGAGAAGCATCTAAGCAGGCAGACGTGAACTTGGTGAACGCTAAGCTACTGGT GAAAGAGGCTGGCCTCAATGTCACCACCTCCCACAACCCTGGGGTTCCAGGGGAGCAGGGTAGCGGGGAA TGCCTCCTGACTGTGGCCCTGGCAGGTGCTCCCTACCAAGCCGTGGGCTTGGTCCAGGGCACCACACCAA TGCTGCAGATGCTCAACGGAGCTGTCTTCAGACCAGAGGTGCCACTACGCAGGGGCCAACCCCTGCTCGT ATTCCGGGCTCAGCCCTCCGACCCTGGGATGCTGCCCACTATGATTGGCCTCCTGGCAGAGGCGGGTGTA CAGCTGCTGTCCTACCAAACCTCCATGGTGTCTGACGGAGAGCCCTGGCACGTCATGGGCCTGTCCTCCC TGCTGCCCAGCCTGGAAACATGGAAGCAGCATGTATTGGAGGCTTTCCAGTTCTGCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_016966 |
Insert Size | 1602 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016966.3, NP_058662.2 |
RefSeq Size | 1871 bp |
RefSeq ORF | 1602 bp |
Locus ID | 236539 |
UniProt ID | Q61753 |
Gene Summary | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Does not catalyze the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224471 | Phgdh (tGFP-tagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh), (10ug) |
CNY 7,560.00 |
|
MR224471 | Phgdh (Myc-DDK-tagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh) |
CNY 5,960.00 |
|
MR224471L3 | Lenti ORF clone of Phgdh (Myc-DDK-tagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh) |
CNY 7,220.00 |
|
MR224471L4 | Lenti ORF clone of Phgdh (mGFP-tagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh) |
CNY 7,220.00 |