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Trim27 (NM_009054) Mouse Untagged Clone
CAT＃: MC217259
Trim27 (untagged) - Mouse tripartite motif-containing 27 (Trim27), (10ug)
CNY 6,650.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW538890; Gm19403; Rfp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217259 representing NM_009054
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCGGGAGCGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTTCAGTACTTCG TGGAGCCTATGATGCTCGACTGCGGCCACAACATCTGTTGCGCGTGCCTGGCCCGCTGCTGGGGTGCCGC GGAGACTAACGTGTCGTGTCCGCAGTGCCGGGAGACCTTCCCGCAGCGCCACATGCGACCCAACCGGCAC CTGGCCAACGTGACCCAGCTGGTGAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGG GCGTGTGCGAGAAGCACCGGGAGCCTCTGAAGCTGTACTGCGAGCAGGACCAGATGCCCATCTGCGTGGT GTGCGACCGCTCGCGCGAGCACCGCGGCCACAGCGTGCTGCCCCTGGAGGAGGCGGTGGAGGGCTTCAAG GAGCAAATCCAGAACCGACTGGACCACCTAAGAAGAGTGAAAGACTTGAAGAAGAGACGGCGGGCACAGG GGGAGCAGGCCCGAGCTGAGCTTTTGAGCCTGACTCAGATGGAGAGGGAAAAAATTGTCTGGGAGTTTGA GCAGCTCTATCACTCGTTGAAGGAGCATGAGTACCGCCTGTTGGCTCGCCTTGAGGAGCTCGACTTGGCC ATCTATAACAGCATCAACGGGGCCATCACTCAGTTCTCCTGCAACATCTCCCACCTCAGTGGCCTGATTG CCCAACTGGAAGAGAAGCAGCAGCAGCCCACGCGGGAGCTCTTACAGGACATTGGAGACACACTGAGCAG GGCTGAAAGAATCAGGATCCCTGAGCCCTGGATCACACCCCCAGATCTACAAGAGAAAATCCATATTTTT GCCCAAAAATGTCTGTTTTTGACAGAAAGTCTAAAACAGTTCACAGAAAAGATGCAGTCAGATATGGAGA AGATCCAAGAGTTGAGAGAGGCTCAGTTATACTCAGTTGATGTAACCCTGGACCCAGACACAGCCTATCC CAGTCTGATTCTTTCTGATAATCTTCGGCAAGTACGGTACAGTTACCTCCAGCAGGATCTTCCAGACAAC CCTGAGCGATTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGTTTCATCGCTGGGAGACATTACTGGG AGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCGGTGTGCAGAAAAGGTGG GGTGACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATGGGAAAGAATATTGGGCTCTT ACATCCCCAATGACTGCACTACCCCTGCGGACCCCTCTCCAGAGAGTAGGGATTTTCTTGGACTATGATG CTGGTGAGGTTTCCTTCTACAATGTGACAGAGAGATGCCATACCTTTACTTTCTCTCACGCTACCTTCTG TGGGCCAGTCCGGCCCTACTTCAGCCTAAGCTACTCGGGAGGGAAGAGCGCAGCTCCTTTGATCATCTGC CCTATGAGTGGGATTGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCTCCGT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009054 |
| Insert Size | 1542 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009054.3, NP_033080.2 |
| RefSeq Size | 4204 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 19720 |
| UniProt ID | Q62158 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208363 | Trim27 (tGFP-tagged) - Mouse tripartite motif protein 27 (Trim27) |
CNY 5,040.00 |
|
| MR208363 | Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27) |
CNY 3,824.00 |
|
| MR208363L3 | Lenti ORF clone of Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27) |
CNY 6,460.00 |
|
| MR208363L4 | Lenti ORF clone of Trim27 (mGFP-tagged) - Mouse tripartite motif-containing 27 (Trim27) |
CNY 6,460.00 |
