Dntt (NM_001043228) Mouse Untagged Clone
CAT#: MC217150
Dntt (untagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2, (10ug)
CNY 6,560.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Tdt |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC217150 representing NM_001043228
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCACTGCAAGCAGTCCACCTGGGTCCTCGGAAGAAAAGACCCAGGCAGCTGGGCACCCCAGTGG CTTCCACGCCTTATGACATCCGATTTCGAGACTTGGTCCTCTTCATTTTGGAAAAGAAGATGGGAACAAC TCGAAGAGCCTTCCTCATGGAGCTGGCCCGAAGGAAAGGGTTCAGGGTGGAAAATGAGCTCAGTGATTCG GTCACCCACATTGTGGCAGAGAACAACTCAGGGTCAGACGTCCTGGAGTGGCTCCAGCTGCAGAACATCA AAGCCAGCTCCGAGCTCGAACTCTTGGACATCTCCTGGCTCATCGAATGCATGGGAGCTGGGAAGCCAGT GGAGATGATGGGGAGACATCAGCTTGTTGTGAATAGAAACTCCTCCCCGAGTCCTGTCCCAGGCTCCCAG AATGTTCCAGCACCTGCTGTGAAGAAGATCTCCCAATATGCTTGCCAGCGAAGAACCACATTAAACAATT ACAACCAACTATTCACGGATGCCCTTGATATCCTGGCTGAAAATGATGAGCTTAGAGAGAATGAAGGTTC TTGCCTGGCATTCATGCGAGCGTCCTCTGTACTGAAATCTCTGCCATTCCCCATCACCAGCATGAAAGAC ACAGAGGGGATTCCTTGCCTAGGGGACAAGGTGAAGAGTATCATAGAGGGAATTATTGAAGATGGAGAAA GTTCTGAAGCTAAAGCTGTGTTAAATGATGAGCGATATAAATCCTTCAAACTCTTTACCTCTGTGTTTGG TGTGGGACTGAAGACAGCTGAGAAATGGTTCAGGATGGGTTTCAGAACTCTCAGCAAAATACAATCAGAC AAAAGCCTGAGGTTTACACAAATGCAGAAAGCAGGATTCCTCTACTACGAAGACCTCGTTAGCTGTGTGA ACAGGCCAGAAGCCGAGGCTGTCAGCATGCTAGTTAAGGAGGCGGTTGTGACATTTCTTCCAGATGCCTT GGTCACCATGACTGGGGGGTTCCGCAGGGGTAAGATGACTGGACATGATGTAGACTTTCTAATTACCAGC CCAGAAGCCACAGAGGATGAAGAGCAGCAGCTCTTGCATAAAGTGACAGACTTTTGGAAGCAGCAGGGGT TGCTTTTGTACTGCGACATCTTAGAGTCAACCTTTGAAAAGTTCAAGCAGCCCAGCAGGAAGGTGGATGC TCTCGACCATTTCCAGAAATGCTTCCTGATTCTGAAGCTGGACCACGGGAGAGTGCACAGCGAGAAGAGC GGCCAGCAGGAAGGAAAGGGCTGGAAGGCCATCCGTGTAGATCTGGTCATGTGCCCCTATGATCGCCGGG CCTTCGCCCTGCTTGGATGGACCGGCTCCAGACAGTTTGAGAGAGACTTGCGGCGCTATGCCACACACGA GAGGAAGATGATGCTGGACAACCACGCCCTGTATGACAGGACTAAGAGGGTGTTTCTTGAAGCAGAAAGT GAAGAAGAGATCTTTGCCCATCTGGGACTGGACTACATTGAACCATGGGAAAGAAATGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001043228 |
Insert Size | 1533 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001043228.1, NP_001036693.1 |
RefSeq Size | 2073 bp |
RefSeq ORF | 1533 bp |
Locus ID | 21673 |
UniProt ID | P09838 |
Gene Summary | Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG221098 | Dntt (tGFP-tagged) - Mouse deoxynucleotidyltransferase terminal (Dntt) transcript variant 2, (10ug) |
CNY 7,672.00 |
|
MR221098 | Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2 |
CNY 5,130.00 |
|
MR221098L3 | Lenti ORF clone of Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2 |
CNY 7,030.00 |
|
MR221098L4 | Lenti ORF clone of Dntt (mGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 2 |
CNY 7,030.00 |