Rrp1 (NM_010925) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL033305; AL033306; AU018604; NNP-1; Nnp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC216808 representing NM_010925 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCCCGGAGTGCCGCTCCCGCCGGAGATCCAGCTGGCGCAGCGCCTGGCGGGCAATGAACAGGTGA CGCGGGACCGCGCGTTGCGGAAGCTGCGGAAGTACATCGAGGCCAGGTCGCAGCGGGCGACAGGTGGTTT CACTCCAGATGAACTGCTAAAAGTATGGAAAGGACTGTTTTACTGCATGTGGATGCAGGACAAGCCCCTC CAGCAGGAAGAACTAGGAAGGACCATTGCCCAGCTTGTCCATGCTTTTCACACGACGGAGGCACAGCACC AGTTCCTGAAGGCCTTCTGGCAGACCATGATCCGGGAGTGGGTGGGCATTGACAGACTGCGCCTGGATAA GTTCTACATGCTCATGAGGATGGTCCTAAGTGAGTCACTGAAGGCTGTGAAAGCCCGGGGATGGGACGAG AGACAGATTGAGCAGCTGCTAGAGCTGCTAACCACCGAGATCCTGAACCCCGACAGCCAGGCCCCCAGTG GGGTGAAGAGCCACTTCCTAGAGATCTTTCTGGAGGAGCTGGCCAAAGTGGGCGCTGCAGAGCTCACCGC AGACCAGAATCTGCAGTTCATCGACCCCTTCTGCCAGATAGCAGCCCGCACTAAGGATTCCCAGGTTTTG CATAAGATCATTCAGAGCATCTTTCAGACGATTGTGGAGCAGGCTCCCTTGGCCATAGAAGACATCATGA ATGAACTGGACACACAGAGTGGAGAGGGTGAAGCATCAGATGGTGATGATGGTGAAGCATCAGATGGTGA TGATGGAGAAGCATCAGACGATGATGATGGTGAAGCGTCAGATGGTGGTGATGGTGATGTGGCAGACAGT GATGATAGTGATGGGGCAGACGATGATGATGGTGACGTGTCAGACGGTGACGGTGGTGACAATGATGAAG GTGACAGTAACAAGTCCTCAGAGGGTGAGCAGGATCTGCAAGACACACCACCCAAGAAGCTGCCTGCAGG CACCGCACATAGGGCTGGCCCTGAGGCAGACAAGGAACAGGCGTGGGATGACGAGGAGAACGCTGGCCCT GTCCTCCAGTTTGACTACGAGGCACTTGCTAACAGGCTGTTCAAGCTCGCCAGTAGACAGAGCACCCCTT CTCAGAACAGGAAGCGCCTCTACAAAGTGATCCAAAAGCTGCGGGAGCTGGCTGGAGGCACTTTCCCAGA GGATGATGTCCCTGAAAAAGCCTACAAGAAGATGCTGGAAGGGAGGCGGGAACGGAAGAAGAAAAAGAAG CGCTTGCCCAAACCTCAGCCCCAGAATAAGGAAGCGGGGAGTGAGGCAGAATCCTCAAGTGCAGACCCAG GCCCAGGAAGGAAGAGGAAGAGGAACAGGAAAACGGATGAGAAGGCTGGCCAAGGGGGTCCTCCTGGGAA GAGGAGGAAGCCCGGGGCTAGGGCAAAGGGGGCTGGGGCCCAGCAACCCAAGAAGAGGATACAGTCATCA CAGAGTGCAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010925
Insert Size	1485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010925.2, NP_035055.2
RefSeq Size	2008 bp
RefSeq ORF	1485 bp
Locus ID	18114
UniProt ID	P56183
Gene Summary	Plays a critical role in the generation of 28S rRNA.[UniProtKB/Swiss-Prot Function]

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