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Ddc (NM_001190448) Mouse Untagged Clone
CAT＃: MC216622
Ddc (untagged) - Mouse dopa decarboxylase (Ddc), transcript variant 1, (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Aadc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216622 representing NM_001190448
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCCGTGAATTCCGGAGGAGAGGCAAGGAGATGGTGGATTATATAGCTGACTATCTGGATGGCA TTGAGGGTCGTCCAGTGTACCCTGATGTGGAGCCTGGCTATCTTCGGCCCCTGATCCCTGCCACTGCCCC CCAGGAGCCAGAAACATACGAGGACATAATCAAAGACATCGAGAAGATAATCATGCCAGGGGTGACACAC TGGCACAGTCCCTATTTCTTCGCTTACTTCCCCACGGCTAGCTCATACCCAGCTATGCTTGCAGACATGC TGTGTGGTGCTATTGGCTGCATTGGTTTCTCCTGGGCTGCAAGCCCAGCGTGCACAGAGCTGGAGACCGT GATGATGGACTGGCTGGGGAAGATGCTGGAGCTGCCAGAGGCCTTTTTGGCTGGAAGAGCTGGGGAAGGG GGAGGAGTGATCCAGGGAAGTGCCAGTGAAGCCACCTTGGTGGCCCTACTGGCTGCTCGGACTAAAGTTA TCCGCCAGCTGCAGGCAGCCTCCCCAGAGTTCACACAAGCTGCTATCATGGAAAAGCTGGTTGCTTACAC ATCTGATCAGGCGCATTCCTCTGTAGAAAGAGCTGGGTTAATTGGTGGAATAAAGCTAAAAGCAGTCCCT TCGGATGGCAACTTTTCCATGAGAGCTTCTGCCCTTCGGGAAGCCCTGGAGCGGGACAAGGCAGCTGGCC TGATTCCATTCTTTGTGGTCGCTACACTGGGGACCACATCCTGCTGTTCTTTTGACAATCTCCTGGAAGT GGGTCCCATCTGCAACCAGGAGGGTGTGTGGCTGCACATTGACGCTGCTTACGCGGGCAGTGCCTTTATC TGTCCTGAATTCCGGTATCTTCTGAATGGTGTGGAGTTTGCAGATTCCTTTAACTTTAATCCCCACAAGT GGCTTTTGGTGAACTTTGACTGCTCTGCCATGTGGGTGAAGAGGAGGACTGACTTAACCGGAGCCTTTAA TATGGACCCTGTTTATCTAAAGCACAGTCACCAGGACTCAGGATTCATCACTGACTACAGGCACTGGCAG ATCCCACTGGGGCGACGATTTCGCTCTTTGAAAATGTGGTTTGTTTTTAGAATGTACGGAGTCAAGGGGC TGCAGGCTTACATCCGAAAGCACGTGGAGCTGTCTCATGAGTTTGAGTCACTGGTACGCCAGGACCCTCG CTTTGAAATTTGCACAGAAGTCATTCTTGGGTTGGTCTGCTTCCGGCTAAAGGGCTCCAATGAGTTGAAC GAAACTCTCTTACAAAGAATAAACAGCGCCAAAAAAATCCACTTGGTTCCATGTCGTCTCCGAGACAAGT TTGTGCTACGCTTTGCTGTGTGCGCTCGCACTGTGGAGTCTGCCCACGTGCAGCTGGCCTGGGAACACAT CAGTGATCTAGCAAGCAGTGTGCTGAGGGCAGAGAAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001190448 |
| Insert Size | 1443 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001190448.1, NP_001177377.1 |
| RefSeq Size | 2018 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 13195 |
| UniProt ID | O88533 |
| Gene Summary | Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226067 | Ddc (tGFP-tagged) - Mouse dopa decarboxylase (Ddc) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
| MR226067 | Ddc (Myc-DDK-tagged) - Mouse dopa decarboxylase (Ddc), transcript variant 1 |
CNY 5,488.00 |
|
| MR226067L3 | Lenti ORF clone of Ddc (Myc-DDK-tagged) - Mouse dopa decarboxylase (Ddc), transcript variant 1 |
CNY 5,230.00 |
|
| MR226067L4 | Lenti ORF clone of Ddc (mGFP-tagged) - Mouse dopa decarboxylase (Ddc), transcript variant 1 |
CNY 5,230.00 |
