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Cpe (NM_013494) Mouse Untagged Clone
CAT＃: MC216440
Cpe (untagged) - Mouse carboxypeptidase E (Cpe), (10ug)
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP; CPH; Cph-; Cph-1; Cph1; fat; NF-a; NF-alpha1; R74677 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216440 representing NM_013494
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGCGCGGAGGACGGGTGCTGCTGGCGCTGTGTGCCGCGCTGGTGGCCGGCGGGTGGCTGCTGA CGGCTGAAGCCCAGGAGCCCGGGGCGCCAGCGGCTGGCATGAGGCGCCGCCGGCGGCTCCAGCAAGAGGA CGGCATCTCCTTCGAGTACCACCGCTATCCAGAGCTGCGCGAGGCGCTGGTGTCCGTATGGCTGCAGTGC ACCGCCATCAGCAGAATCTACACAGTGGGGCGCAGCTTCGAGGGCCGGGAGCTCCTGGTCATCGAGCTGT CTGACAACCCCGGGGTCCATGAGCCGGGTGAACCTGAATTTAAATACATTGGGAACATGCATGGTAATGA GGCGGTTGGACGGGAACTGCTTATTTTCTTGGCCCAGTACCTGTGTAACGAGTACCAGAAAGGCAATGAG ACAATTGTCAACCTGATCCACAGCACCCGAATTCATATCATGCCCTCCTTGAACCCCGACGGCTTTGAGA AAGCCGCATCGCAGCCCGGCGAGCTGAAGGACTGGTTTGTGGGCCGCAGCAACGCCCAGGGAATAGATCT GAACCGTAACTTCCCAGACCTGGACAGGATCGTGTATGTTAATGAGAAAGAAGGCGGTCCTAACAATCAC CTGCTGAAGAATCTGAAGAAAATTGTGGACCAAAATTCAAAGCTTGCCCCCGAGACCAAGGCTGTCATTC ACTGGATCATGGACATTCCATTTGTGCTTTCTGCCAATCTGCACGGAGGAGACCTTGTGGCTAATTACCC ATATGATGAGACACGGAGCGGTACTGCTCACGAATACAGTTCCTGCCCTGATGACGCAATTTTCCAAAGC TTGGCTCGCGCGTACTCTTCTTTCAACCCAGTCATGTCTGACCCCAATCGACCTCCCTGTCGCAAGAATG ACGATGACAGCAGCTTTGTAGATGGAACGACCAATGGTGGTGCATGGTACAGCGTCCCCGGTGGAATGCA AGACTTCAATTACCTGAGCAGCAACTGCTTCGAGATCACTGTGGAGCTTAGCTGTGAGAAGTTCCCACCG GAAGAGACTCTCAAAAGCTACTGGGAAGATAACAAAAACTCCCTCATCAGCTACCTGGAGCAGATACACC GAGGTGTTAAAGGGTTTGTCCGTGACCTTCAGGGTAACCCGATTGCCAACGCAACCATCTCTGTGGACGG GATAGACCATGATGTCACCTCGGCTAAGGATGGGGATTACTGGCGATTGCTTGCTCCTGGAAACTATAAA CTTACAGCCTCCGCTCCTGGCTACCTGGCAATCACAAAGAAAGTGGCAGTTCCTTTTAGCCCTGCTGTTG GGGTGGACTTTGAGCTTGAGTCTTTCTCTGAAAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTG GAAAATGATGTCAGAAACTTTGAATTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013494 |
| Insert Size | 1431 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013494.4, NP_038522.2 |
| RefSeq Size | 2183 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 12876 |
| UniProt ID | Q00493 |
| Gene Summary | This gene encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226559 | Cpe (tGFP-tagged) - Mouse carboxypeptidase E (Cpe), (10ug) |
CNY 4,560.00 |
|
| MR226559 | Cpe (Myc-DDK-tagged) - Mouse carboxypeptidase E (Cpe) |
CNY 3,656.00 |
|
| MR226559L3 | Lenti ORF clone of Cpe (Myc-DDK-tagged) - Mouse carboxypeptidase E (Cpe) |
CNY 6,080.00 |
|
| MR226559L4 | Lenti ORF clone of Cpe (mGFP-tagged) - Mouse carboxypeptidase E (Cpe) |
CNY 6,080.00 |
