Nr0b1 (NM_007430) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AH; Ahc; Ahch; AHX; Dax; DAX-; DAX-1; Dax1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC216429 representing NM_007430 Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTGAGGACCACCCGTGGCAGGGCAGCATCCTCTACAATCTACTGATGAGCGCGAAGCAGAAGC ACGCGTCTCAGGAAGAGCGAGAGGTGCGCTTGGGGGCTCAGTGCTGGGGTTGCGCCTGCGGTGCTCAGCC CGTCCTGGGTGGGGAGAGACTGTCCGGCGGGCAAGCCAGGTCCCTCTTGTACCGCTGCTGCTTTTGTGGG GAGAATCACCCGCGCCAGGGTGGCATCCTCTACTCTATGCTCACCAACGCCAGGCAGCCAAGCGTGGCGA CCCAGGCGCCGAGGGCACGATTCGGAGCACCTTGCTGGGGCTGCGCCTGCGGCAGCGCAGAGCCCCTGGT GGGCAGAGAGGGGCTGCCGGCTGGCCAGGCCCCCTCGCTCCTGTACCGCTGCTGCTTCTGCGGAGAAGAG CACCCGAGGCAGGGCAGCATCTTATACAGCTTGCTCACTAGCGCTCAGCAAACGCACGTGTCTCGGGAAG CACCCGAGGCACATCGCAGAGGCGAGTGGTGGCAGCTGTCCTACTGTACCCAGAGTGTGGGTGGCCCAGA GGGGCTGCAGAGCACACAGGCCATGGCGTTCCTGTACCGCAGCTATGTGTGCGGTGAAGAGCAGCCCCAG CAGATCAGCGTTGCCTCTGGCACGCCCGTGAGCGCAGACCAAACACCAGCGACCCCGCAAGAGCAGCCGA GGGCTCCCTGGTGGGACGCCTCACCTGGTGTGCAGCGTCTGATCACACTCAAGGATCCACAGGTGGTGTG CGAGGCAGCGTCCGCTGGCCTGTTGAAGACCCTGCGCTTTGTCAAGTACTTGCCCTGCTTCCAGATCCTG CCCCTAGATCAGCAGCTGGTGCTGGTGCGGAGCTGTTGGGCGCCCCTACTCATGCTTGAGTTGGCCCAAG ATCACCTGCACTTCGAGATGATGGAGATCCCGGAGACCAACACGACGCAGGAAATGCTTACCACCAGGCG GCAGGAGACCGAAGGTCCAGAGCCTGCAGAGCCCCAGGCCACAGAGCAGCCACAGATGGTGTCCGCGGAG GCTGGGCACTTGCTCCCAGCTGCTGCGGTCCAGGCCATCAAGAGTTTCTTTTTCAAGTGCTGGAGTCTGA ACATTGACACCAAAGAGTATGCCTATCTGAAAGGGACCGTGCTCTTTAACCCAGACCTGCCTGGCCTGCA GTGCGTGAAATACATTGAGGGTCTTCAGTGGAGAACCCAGCAGATCCTTACTGAGCACATCCGGATGATG CAGAGAGAGTACCAGATCAGATCCGCTGAACTGAACAGTGCCCTTTTCCTGCTGAGATTCATCAATAGCG ATGTCGTCACTGAACTCTTTTTCAGGCCCATCATTGGTGCAGTCAGCATGGATGATATGATGCTGGAGAT GCTCTGTGCAAAGCTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII
ACCN	NM_007430
Insert Size	1419 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007430.4, NP_031456.1
RefSeq Size	1794 bp
RefSeq ORF	1419 bp
Locus ID	11614
UniProt ID	Q61066
Gene Summary	This gene encodes an orphan nuclear receptor protein that plays a key role in differentiation of the gonads. This protein regulates steroidogenic factor 1 (Sf-1) in a dose-dependent manner, sometimes functioning as a repressor of SF-1 target genes, and sometimes functioning as a co-activator. Overexpression of this gene can cause feminization of the XY male gonads. This gene is also involved in the maintenance of embryonic stem cell pluripotancy. Mutations in the related gene in human cause congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, May 2015]

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