Ero1lb (NM_026184) Mouse Untagged Clone

CAT#: MC216227

Ero1lb (untagged) - Mouse ERO1-like beta (S. cerevisiae) (Ero1lb), (10ug)



  "NM_026184" in other vectors (5)

CNY 1,200.00

CNY 6,080.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1300013B24Rik; 1700065B09Rik; AI447560; ero1-beta; Ero1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216227 representing NM_026184
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCCGGGGTTCCGCCGGGCCGTTACTGGGCAGGGGGCGGCGGCCGCGGTGCAACTGCTTGTCACCC
TGAGCTTCCTCTCAAGTCTGGTCAAGACTCAGGTGACTGGAGTTCTGGATGATTGCTTATGTGACATTGA
CAGCATTGATAAATTCAACACCTACAAAATCTTTCCCAAAATAAAGAAGTTACAAGAACGAGACTATTTT
CGTTATTACAAGGTTAATCTGAAACGACCATGTCCTTTCTGGGCAGAAGATGGCCACTGCTCAATAAAAG
ACTGTCATGTGGAGCCCTGTCCAGAAAGTAAAATTCCAGTTGGAATTAAAGCCGGGCGTTCAAATAAGTA
CTCGCAAGCAGCAAACAGCACCAAAGAACTGGATGACTGTGAGCAGGCTAACAAACTGGGCGCCATCAAC
AGCACGCTAAGTAACGAAAGCAAAGAAGCGTTCATTGACTGGGCGAGATATGATGATTCGCAGGACCACT
TTTGTGAACTTGATGATGAGCGGTCTCCTGCTGCACAGTATGTGGACCTGCTGCTGAACCCGGAACGGTA
CACTGGCTACAAGGGCTCCTCAGCATGGAGGGTGTGGAACAGCATCTATGAAGAAAACTGCTTCAAGCCT
CGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGATGGAGAATCATTCTATA
CGTGGCTAGAAGGTTTGTGTCTTGAGAAAAGAGTCTTCTATAAGCTTATATCAGGACTCCATGCCAGCAT
CAATTTACATCTGTGTGCAAACTACCTTCTGGAAGAAACCTGGGGGAAACCTAGTTGGGGACCAAACATC
AAGGAGTTTAGACGCCGCTTTGACCCTGTGGAAACAAAGGGGGAAGGTCCAAGGAGGCTAAAGAACCTGT
ACTTTTTATACTTGATAGAGCTCCGTGCTTTGTCAAAGGTGGCCCCTTACTTTGAGCGCTCGATTGTTGA
TCTCTATACTGGCAATGTGGAAGATGATGCCGACACCAAGACCCTTCTGCTCAGCATCTTTCAGGATACA
AAGTCCTTTCCTATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAA
AGGAAGAATTCCGGTTACATTTCAAGAACATCTCCCGGATCATGGACTGTGTTGGGTGCGATAAATGCAG
ACTGTGGGGGAAACTGCAGACTCAGGGTTTAGGAACTGCCTTGAAGATCCTCTTCTCTGAAAAGGAAATC
CAAAACCTTCCGGAGAACAGCCCATCCAAAGGCTTCCAGCTCACTCGGCAGGAAATCGTTGCTCTTTTAA
ATGCTTTTGGAAGACTTTCTACAAGCATAAGAGAATTACAGAACTTTAAAGCGTTGTTGCAGCACAGGAG
GTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_026184
Insert Size 3 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058721, AAH58721
RefSeq Size 4255 bp
RefSeq ORF 1404 bp
Locus ID 67475
UniProt ID Q8R2E9
Gene Summary Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2, PDIA3, PDIA4, PDIA6 and NXNDC12. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity). Involved in oxidative proinsulin folding in pancreatic cells, hence required for glucose homeostasis in vivo.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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