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Espn (NM_207689) Mouse Untagged Clone
CAT＃: MC215988
Espn (untagged) - Mouse espin (Espn), transcript variant 4, (10ug)
CN¥ 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | je |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215988 representing NM_207689
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAATAGCTTGGAACACCGAGTCTTGTCCCGGGATCAATCCATGGACCTGGAGGCAAAGCAGCTGG ACTCGGGTATGTCCTCGCCCAACACCACCATGTCGGTCCAGCCAATGACCTTTGACCTGGGCTCGCCTAC TAGCACGTTCTCCAACTATGACTCCTGCTCCTCCAGTCACTCCAGCAGCAAGGGGCAGCGATCGAATCGA GGGATTCCAGGTGCAAGAGCTGCAGACTTACAGAGCTACATGGACATGCTGAACCCAGAGAAGAGCTTGC CTCGGGGCAAGCTAGGGAAGCCTTCCCCGCCACCACCTCCACCACCACCACCACCAAGCTTCCCGCCACC CCCACCACCCACAGGCACCCAGCCGCCCCCACCTCCACCAGGCTACCCAGCTCCCAATCCCCCTGTGGGA CTGCATCTGAATAACATTTACATGCAGACCAAGAACAAGCTTCGCCATGTGGAGGTGGACTCGCTCAAGG AGGCGGCGCTGCTCCCCGGGAACCACGTGCACAACGGCTGCTCAGCGGACTCCAAAGCGTCCAGGGAGCT GCCGCCGCCACCGCCGCCGCCGCCGCTGCCCGAGGCCCTGAGTTCGCCGCCGCCCGCCCCACCTCTGCCC ATCGAGGGCGCGGGCGCAGCCTGCGGGCAGCGTCGTTCCTCGTCTTCTACTGGCAAAGTGAGAGTCCTGA GACACAGGAAGAGCACCAAATCTTTCAACATGATGTCCCCAACGGGTGATAACTCAGAGCTTCTGGCTGA GATAAAGGCGGGCAAGAGCCTGAAGCCGACACCGCAGAGCAAGGGGCTGACAACCGTGTTCTCAGGCAGT GGGCAGCCAGCCTCCCAGCCTGAGTCACCGCAGCCTCTGGTGTCACCTGCGCCATCTCGGACTCGGAGCC CCACCCCGCCAGCCTCTGGGTCTCAGCCACTGCTCAATGGCAGTGTGGTGCCGGCACCACCTGCCACCCC GGCACCTGGAGTCCATCTGGATGTGGAGGCCCTCATTCCCACTCTTGATGAGCAGGGCCGGCCCATCCCG GAGTGGAAGCGCCAGGTGATGGTCCGCAAGCTGCAGCAGAAGATGCAGGAGGAAGAGGAGCAGCGGAGGA AGGAGGAAGAGGAGGAGGCCCGGCTCGCCAGCCTGCCTGCCTGGAGACGAGACATTCTTCGGAAGAAGCT GGAGGAGGAGAGGGAGCAGAAGCGAAAAGAGGAGGAGCGGCAAAAGCTGGAGGAAATACAGAGGGCGAAA GAACAGTCGGAGAAGCTGCGGACACTAGGCTACGACGAAGCCAAGCTCGCGCCCTGGCAGCGACAGGTCA TCTTGAAGAAGGGGGAGATCCCTAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_207689 |
| Insert Size | 1359 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_207689.2, NP_997572.1 |
| RefSeq Size | 1647 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 56226 |
| UniProt ID | Q9ET47 |
| Gene Summary | Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells (PubMed:14657236, PubMed:15190118). Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia (PubMed:21455486). Involved in the elongation of actin in stereocilia (PubMed:19287378, PubMed:22264607). In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation (PubMed:26926603).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 4 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no full-length transcript from the C57BL/6J reference strain was available. The extent of this transcript is supported by its existence in a single strain, CBA/CaJ. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224956 | Espn (tGFP-tagged) - Mouse espin (Espn) transcript variant 4, (10ug) |
CN¥ 4,940.00 |
|
| MR224956 | Espn (Myc-DDK-tagged) - Mouse espin (Espn), transcript variant 4 |
CN¥ 4,470.00 |
|
| MR224956L3 | Lenti ORF clone of Espn (Myc-DDK-tagged) - Mouse espin (Espn), transcript variant 4 |
CN¥ 6,370.00 |
|
| MR224956L4 | Lenti ORF clone of Espn (mGFP-tagged) - Mouse espin (Espn), transcript variant 4 |
CN¥ 6,370.00 |
