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Polr1e (NM_022811) Mouse Untagged Clone
CAT＃: MC215729
Polr1e (untagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e), (10ug)
CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 53kDa; AU042259; D030019D19Rik; Paf53; Praf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215729 representing NM_022811
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG AGTCACCGGATCGTGGCAGCTGAAACAGATAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGACGATGCTATTGAGCGAGAGCCTCCTCTGGAG AATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAGAAGC GGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAGCCGC GGAGAGTATTATTGATACAAAGGGTGTGAATGCGCTGGTCAGCGATGCCATGCAGGATGACTTACAAGAT GGCGTCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAAGACA TTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGGAAGA CATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCCCATA GATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGAGCTC AGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGCTGAA ACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTCATTTCAAGTTCTATGAGGGCCAAG ATCACTTCGTATGCGATTATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTGCAGA AGGACTTGAAGCTGAGTGAGAAAAGGATGATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCAAACA AAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCCTCCA GCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022811 |
| Insert Size | 1305 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022811.3, NP_073722.1 |
| RefSeq Size | 3547 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 64424 |
| UniProt ID | Q8K202 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two alternate exons that result in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219108 | Polr1e (tGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e), (10ug) |
CNY 4,850.00 |
|
| MR219108 | Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e) |
CNY 4,370.00 |
|
| MR219108L3 | Lenti ORF clone of Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e) |
CNY 6,270.00 |
|
| MR219108L4 | Lenti ORF clone of Polr1e (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e) |
CNY 6,270.00 |
