Ap2m1 (NM_009679) Mouse Untagged Clone

CAT#: MC215697

Ap2m1 (untagged) - Mouse adaptor protein complex AP-2, mu1 (Ap2m1), (10ug)



  "NM_009679" in other vectors (4)

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215697 representing NM_009679
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTTATCTCCCGGGTCTACAGAGATGACA
TCGGGAGGAATGCTGTGGATGCCTTTCGGGTCAATGTCATTCATGCACGGCAGCAGGTGCGCAGCCCTGT
CACAAACATCGCTCGCACCAGCTTCTTCCATGTTAAGCGGTCCAACATCTGGCTGGCCGCAGTCACCAAG
CAGAATGTCAATGCTGCCATGGTCTTCGAATTCCTCTACAAGATGTGTGATGTAATGGCTGCTTACTTTG
GCAAAATCAGCGAGGAGAACATCAAGAACAATTTTGTGCTCATATACGAGCTGCTGGATGAGATTCTGGA
CTTTGGCTACCCACAGAACTCAGAGACAGGTGCACTGAAAACCTTCATCACCCAGCAGGGTATCAAGAGT
CAGCATCAGACGAAGGAAGAACAGTCCCAGATCACCAGCCAGGTGACCGGGCAGATTGGCTGGCGGCGAG
AAGGCATCAAGTATCGCCGGAATGAACTCTTCCTAGATGTTCTGGAGAGTGTGAACCTGCTTATGTCCCC
ACAGGGGCAGGTGCTGAGTGCCCATGTGTCAGGCCGGGTGGTGATGAAGAGTTACCTGAGTGGCATGCCT
GAGTGCAAGTTTGGAATGAATGACAAGATTGTCATAGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAA
GCAAGAGTGGTAAGCAGTCGATCGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTT
TGACTCTGAGCGGAGCATCAGCTTCATCCCTCCCGACGGAGAGTTTGAACTCATGAGATACCGTACTACC
AAGGACATCATCCTTCCTTTCCGGGTGATCCCATTGGTGCGGGAGGTGGGGCGCACCAAACTGGAGGTCA
AGGTGGTCATCAAGTCCAACTTCAAGCCCTCACTTCTGGCCCAGAAGATTGAGGTGAGGATCCCGACTCC
ATTGAACACAAGCGGGGTACAGGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAACGCC
ATTGTATGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCCCAGATCAGCGCAGAGATTGAGCTCCTGC
CCACCAACGATAAGAAGAAATGGGCTCGGCCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCTTC
TGGCCTCAAGGTGCGCTACTTGAAGGTATTCGAACCGAAGCTGAACTACAGCGACCATGATGTCATCAAA
TGGGTGCGATACATTGGCCGCAGTGGCATTTATGAAACCCGCTGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009679
Insert Size 1308 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009679.3, NP_033809.1
RefSeq Size 2937 bp
RefSeq ORF 1308 bp
Locus ID 11773
UniProt ID P84091
Gene Summary This gene encodes the mu subunit of the clathrin adaptor protein complex AP-2. It mediates sorting of cargo proteins harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate splicing of this gene results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2, 8 and 19. [provided by RefSeq, Dec 2014]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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