Gfral (NM_205844) Mouse Untagged Clone

CAT#: MC214864

Gfral (untagged) - Mouse GDNF family receptor alpha like (Gfral), (10ug)



  "NM_205844" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AY457637; Gral
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC214864 representing NM_205844
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTAGTGTTCATTTTCCTGGCTGTTACGTTAAGCTCAGAAAATGAATCCTCTTCCCAAACAAATGATT
GTGCACATTTAATACAGAAATGCTTGATTGATGCAAATGGCTGTGAGCAGTCATGGAGATCAATGGAAGA
CACCTGCCTTACTCCAGGTGACTCCTGCAAGATAAATAATTCACTACATTGTAACCTGAGTATCCAGGCT
TTGGTGGAAAAAAATTTCCAATTTAAAGAGTGTCTTTGTATGGATGACCTCCACTGTACAGTAAACAAAC
TTTTTGGAAAAAAGTGCACCAATAAGACAGATAACATGGAAAAGGACAATAAAGATAAATGGAATCTAAC
TACTACTCCTTTCTATCATGGATTCAAACAGATGCAGTCTTGTTTGGAGGTGACAGAGGCGTGTGTAGGG
GATGTGGTTTGTAATGCACAGTTGGCCCTTTACCTTAAAGCATGCTCAGCAAATGGAAATCTGTGTGATG
TGAAACACTGCCAAGCAGCCATACGGTTCTTCTATCAAAATATGCCTTTTAACACTGCCCAGATGTTGGC
TTTTTGTGACTGTGCTCAATCTGATATACCCTGTCAGCAATCCAAAGAAACTCTTCACAGCAAGCCATGT
GCACTGAATATAGTTCCACCCCCCACTTGCCTCAGTGTAATTCACACTTGCCGAAATGATGAATTATGCA
GGACACACTACCGAACATTCCAGACAGAATGCTGGCCCCACATAACTGGGAAGTGCCATGAAGATGAGAC
CTGCATTAGCATGTTAGGCAAGCAAGACCTTACTTGTTCTGGGAGTGAGAGCTGCAGGGCTGCCTTCCTA
GGAACCTTTGGGACAGTCCTGCAAGTACCCTGTGCTTGCAGGGGCGTTACACAGGCTGAAGAACACGTGT
GCATGATTTTCCAGCACATGCTTCATAGCAAATCGTGTTTCAATTACCCAACTCCTAATGTCAAAGACAT
TTCCTCATATGAAAAAAAGAATTCAAAAGAAATTACTCTGACTGGATTCAATTCTTTCTTCAATGGAGAA
CTACTCTATGTTGTTGTGTGCATGGCAGTTACCTGTGGAATTCTTTTCTTGGTGATGCTCAAGTTAAGGA
TACAAAGTGAAAAAAGAGATCCCTCATCCATCGAAATAGCTGGAGGTGTCATCATTCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_205844
Insert Size 1182 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_205844.3, NP_995316.2
RefSeq Size 2080 bp
RefSeq ORF 1182 bp
Locus ID 404194
UniProt ID Q6SJE0
Gene Summary Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846099, PubMed:28846097, PubMed:28846098). Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways (PubMed:28846098, PubMed:28846099).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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