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Prmt8 (NM_201371) Mouse Untagged Clone
CAT＃: MC214730
Prmt8 (untagged) - Mouse protein arginine N-methyltransferase 8 (Prmt8), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Hrmt1l3; Hrmt1l4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC214730 representing NM_201371
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTCCGGAGGAAAATGGCGGAGAATGCAGTCGAAAGCA CCGAGGTGAGCAGCGCCCCCCCTCAGCCCCCTCAGCCTGTCATTCCAGCCAAGCCTGTGCAATGTGTCCA CCATGTATCCACACAGCCCAGCTGCCCTGGACGGGGCAAGATGTCCAAGCTGCTGAACCCCGAGGAGATG ACATCGAGAGATTACTATTTCGACTCATACGCTCACTTTGGGATCCACGAGGAAATGTTGAAAGACGAGG TGAGGACACTCACGTACCGGAACTCCATGTACCATAATAAGCATGTGTTCAAGGACAAAGTGGTACTGGA TGTTGGGAGTGGCACAGGGATCCTTTCCATGTTTGCCGCCAAAGCAGGGGCCAAGAAGGTGTTTGGGATT GAATGTTCCAGTATTTCTGACTACTCGGAGAAGATCATTAAGGCCAATCACCTGGACAATGTCATCACCA TATTTAAGGGCAAAGTGGAGGAGGTGGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGAT GGGCTACTGTCTGTTCTACGAGTCCATGCTCAACACCGTGATCTTTGCCAGGGACAAGTGGTTGAAACCT GGAGGGCTTATGTTTCCAGACCGGGCAGCTTTGTACGTGGTAGCAATCGAAGACAGACAGTACAAGGACT TCAAAATCCACTGGTGGGAGAATGTCTATGGCTTTGACATGACCTGCATCCGGGATGTTGCCATGAAGGA GCCCCTGGTGGACATCGTGGACCCAAAGCAAGTGGTGACCAATGCCTGTTTAATAAAGGAAGTGGACATT TACACTGTGAAGACGGAAGAGCTGTCCTTCACGTCTGCCTTCTGCCTGCAGATCCAGCGCAACGACTATG TCCATGCCCTCGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAGAAAATGGGTTTTTCTACAGC CCCTGATGCCCCCTACACCCACTGGAAGCAGACTGTCTTCTACTTAGAAGACTACCTCACTGTCCGGAGA GGGGAGGAAATCTACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAG TAGACTTGGATTTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_201371 |
| Insert Size | 1185 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_201371.2, NP_958759.2 |
| RefSeq Size | 2413 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 381813 |
| UniProt ID | Q6PAK3 |
| Gene Summary | S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer. Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205920 | Prmt8 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 8 (Prmt8) |
CNY 4,370.00 |
|
| MR205920 | Prmt8 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 8 (Prmt8) |
CNY 5,488.00 |
|
| MR205920L3 | Lenti ORF clone of Prmt8 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 8 (Prmt8) |
CNY 5,890.00 |
|
| MR205920L4 | Lenti ORF clone of Prmt8 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 8 (Prmt8) |
CNY 5,890.00 |
