Ksr2 (NM_001034873) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC214619 representing NM_001034873 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGGAAAACATGACGAAAAGCGAAGAGCAGCAACCTCTGAGTTTGCAAAAAGCCTTGCAGCAGT GCGAGTTGGTTCAGAACATGATCGACCTGAGCATCTCCAATCTGGAAGGGCTTAGGACCAAGTGTGCAGC TTCCAACGACCTCACGCAGAAGGAGATCAGGACCCTGGAGAGCAAGCTGGTGAAGTACTTCAGCCGACAG CTGTCCTGCAAGAAGAAGGTGGCTCTGCAGGAACGCAATGCTGAACTGGACGGCTTCCCCCAGCTCCGCC ACTGGTTCCGGATCGTGGATGTCCGAAAGGAAGTCCTGGAGGAGATCTCCCCTGACCAGCTGAGCTTGGA GGACCTCCTGGAGATGACCGACGAGCAGGTGTGTGAGACCGTGGAGAAGTATGGAGCCAACCAGGAAGAG TGCGCCCGGCTCAACGCCTCACTCTCCTGCCTCAGGAATGTGCACAAATCAGGAGGGAATCTCTCCAAAC AAGATTGGATCATCCAGTGGCCCACCACGGAGCCAGGGCAGGAGAGCAACCCTGTGTGCCCCCCGGAGCC CAGCCCGTGGATCCGAACCCACCTCTCCCAGAGCCCCAGGGTCCAGACCAAGTGTCCCCAGCACTTCTGT CCTACCAGCCCTACGCCTGGGACCCCTGTGTACACCCAAGTGGACAGACTCACTGTAGATGCCTACCCGA ACTTGTGCCCGCCGCCGCCACCCCTGGAATCAGGCCACCGCTCTCTGCCCCCGTCTCCCCGGCAGCGGCA CGTAGTCCGCACCCCACCTCGCACCCCAAATATTGTCACCACCGTGACCCCACCTGGCACGCCACCCATG AGGAGAAAAAACAAGCTGAAGCCTCCCGGGACGCCACCCCCCTCCTCTCGGAAGCTCATCCATCTGATCC CTGGCTTCACAGCGCTGCACAGGAGCAAATCCCACGAGTTCCAGCTCGGGAACCGTGTAGACGAGGCCAA CACACCCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001034873
Insert Size	993 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001034873.2, NP_001030045.1
RefSeq Size	2283 bp
RefSeq ORF	993 bp
Locus ID	333050
Gene Summary	Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production (By similarity).[UniProtKB/Swiss-Prot Function]

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