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Zfyve27 (NM_001164531) Mouse Untagged Clone
CAT＃: MC214422
Zfyve27 (untagged) - Mouse zinc finger, FYVE domain containing 27 (Zfyve27), transcript variant 2, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2210011N02Rik; 9530077C24Rik; AI426636; AI593546; AI835681 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC214422 representing NM_001164531
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACTTCGGATCGGGACCTGAGTGGGCCGGAGGCGAGCCCCAGCGGGATGCCTGAGGTTCTCTCTG AGTGTCCACCTGCCCCTACCAAGTCAGCAGCGTTTGATCTCTTCAACCTGGTCCTGTCCTACAAGAGGCT GGAGATCTACCTGGAACCCCTGAAGGATGCAGGGGACGGTGTTCGATACTTGCTAAGGTGGCAGATGCCT TTGTGTTCCTTGCTGACTTGCCTGGGCCTCAACATCTTGTTCCTCACTTTGAACGAGGGTGCGTGGTACT CCATGGGTGCCTTGATGATCTCGGTGCCTGCCCTACTGGGCTACCTTCAGGAGGTGTGCCGGGGACAGCT GCCAGAGTCGGAGCTGATGCGGAGGAAGTACCACAGCATAAGGCAGGAAGACCTGCAGAGAGTTCGCCTT TCCCGAGTGCACCTTTCCCGCCCTGAGGCTGTTGCTGAGGTGAAGAGCTTCTTGATCCAACTGGAAGCCT TCTTGGCCCGCCTGTGCTATACCTGCGAGTCAGCCTACCGTGTACTTCACTGGGAGAACCCCGTGGTGTC CTCACAGTTCTATGGCGCTCTTCTGGGCATGGTGTGCATGCTCTACCTGCTACCGCTCTGCTGGGTCCTC GCCCTGTTAAATAGCACACTCTTCCTGGGAAATGGGGACTTCTTCCGAGTGGTGTGTGAGTACAGGGCCT GCCTGCAGCGGAGGATGAACCCCCGGCAGGAAGAGTGTGCCTGTGAGAGCTCAGCACTGCAGGGGGCCGG AGGGAGGGGCCTGCTGGACAGCTCGCCTGCCCCCACACCCACCGAGGACCTCACGCCAGGCAGTGTGGAG GAAGCTGAGGAGGCTGAGCCAGATGAGGAGTTCAAAGATGCAATTGAGGAGGATGAAGAGGGCACCCCGT GCCCAGCAGAGGATGAGCTGACCCTGCAGGACAATGGATTCCTCAGCAAGAATGAGGTGCTGCGCAGCAA GGTGTCCCGGCTTACAGAGCGGCTTCGCAAGCGTTACCCAACCAATAACTTCGGGAATTGTGCAGGCTGT GCCGCTACGTTCTCCGTGCTGAAGAAGAGGCGGAGCTGCAGCAACTGTGGGAACAGCTTCTGCTCGCGGT GTTGCTCCTTCAAGGTGCCCAGGTCCTCTATGGGAGCCACAGCTCCTGAAGCCCAGAGAGAGACTGTGTG TGTGTGCGCCTCCTGCAATCAGACCCTGAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001164531 |
| Insert Size | 1227 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001164531.1, NP_001158003.1 |
| RefSeq Size | 5498 bp |
| RefSeq ORF | 1227 bp |
| Locus ID | 319740 |
| UniProt ID | Q3TXX3 |
| Gene Summary | Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (By similarity). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (PubMed:24251978). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (By similarity). Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed:21976701).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217010 | Zfyve27 (tGFP-tagged) - Mouse zinc finger FYVE domain containing 27 (Zfyve27) transcript variant 2, (10ug) |
CNY 4,470.00 |
|
| MR217010 | Zfyve27 (Myc-DDK-tagged) - Mouse zinc finger, FYVE domain containing 27 (Zfyve27), transcript variant 2 |
CNY 4,090.00 |
|
| MR217010L3 | Lenti ORF clone of Zfyve27 (Myc-DDK-tagged) - Mouse zinc finger, FYVE domain containing 27 (Zfyve27), transcript variant 2 |
CNY 5,990.00 |
|
| MR217010L4 | Lenti ORF clone of Zfyve27 (mGFP-tagged) - Mouse zinc finger, FYVE domain containing 27 (Zfyve27), transcript variant 2 |
CNY 5,990.00 |
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