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Lclat1 (NM_001177968) Mouse Untagged Clone
CAT＃: MC212744
Lclat1 (untagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 3, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Agpat8; AI181996; Alcat1; Gm91; Lycat |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC212744 representing NM_001177968
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCATGGAAGGGGATTTACTTTATACTCTTCCTGTTTGCTGGAAGCTTTTTTGGAAGTATTTTTA TGCTCGGCCCCATTTTACCTTTGATGTTTATAAACCTGTCGTGGTATCGCTGGATTAGCAGCCGTCTTGT GGCTACATGGCTCACACTTCCTGTGGCATTGCTGGAGACCATGTTTGGTGTGAGAGTGGTTATAACAGGT GACGCCTTTGTGCCTGGAGAGCGGAGCGTCATCATCATGAACCACCGGACACGTGTGGACTGGATGTTCC TGTGGAACTGTCTAATGAGGTACAGCTACCTCAGGGTGGAGAAGATTTGCCTCAAATCCAGTCTCAAAAG TGTTCCTGGATTCGGCTGGGCCATGCAAGTTGCGGCCTTTATCTTTATTCATAGGAAGTGGAAGGATGAT AAGAGCCATTTTGAAGACATGATTGATTATTTTTGTGCCATCCATGAACCACTACAGCTTCTCATTTTTC CAGAAGGAACTGACCTCACAGAAAATAATAAGGCTAGGAGTAATGATTTTGCTGAGAAGAACGGACTTCA GAAATATGAGTATGTTTTACACCCAAGAACCACTGGCTTTACCTTTGTGGTGGACCGCCTAAGAGAAGGG AAGAACCTCGATGCTGTTCATGACATCACGGTCGCATATCCTTACAACATCCCTCAAACTGAGAAGCACC TTCTCCTTGGAGACTTTCCCAAGGAGATCCACTTCCACGTCCAGCGGTATCCAGCTGACTCTCTTCCCAC ATCCAAGGAGGACCTTCAGCTCTGGTGCCACAGAAGGTGGGAAGAAAAGGAGGAGAGGCTTCGGTCCTTC TACCAAGGAGAGAAAAACTTCCACTTTACTGGGCAGAGTACAGTTCCACCTTGCAAGTCTGAGCTCAGAG TCCTTGTGGTCAAGCTACTGTCCATAGTGTACTGGGCCTTGTTCTGCTCTGCAATGTGCCTGCTCATATA TCTGTACAGCCCTGTTCGGTGGTATTTTATAATCAGCATTGTGTTCTTCGTGCTGCAGGAGAGAATATTT GGTGGACTGGAAATCATTGAACTTGCATGTTACCGTTTTTTACACAAGCACCCACATTTAAATTCAAAGA AAAATGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177968 |
| Insert Size | 1131 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177968.1, NP_001171439.1 |
| RefSeq Size | 4444 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 225010 |
| UniProt ID | Q3UN02 |
| Gene Summary | Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (PubMed:15152008). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (PubMed:15152008). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Possesses lysophosphatidylinositol acyltransferase (LPIAT) activity (PubMed:20668164). Possesses lysophosphatidylglycerol acyltransferase (LPGAT) activity (By similarity). Required for establishment of the hematopoietic and endothelial lineages (PubMed:17675553).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226119 | Lclat1 (tGFP-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1) transcript variant 3, (10ug) |
CNY 3,140.00 |
|
| MR226119 | Lclat1 (Myc-DDK-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 3 |
CNY 3,656.00 |
|
| MR226119L3 | Lenti ORF clone of Lclat1 (Myc-DDK-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 3 |
CNY 4,750.00 |
|
| MR226119L4 | Lenti ORF clone of Lclat1 (mGFP-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 3 |
CNY 4,750.00 |
