A3galt2 (NM_001009819) Mouse Untagged Clone

CAT#: MC212623

A3galt2 (untagged) - Mouse alpha 1,3-galactosyltransferase 2 (isoglobotriaosylceramide synthase) (A3galt2), (10ug)



  "NM_001009819" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gm433; iGb3; iGb3S
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212623 representing NM_001009819
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGGGGACAGAGTTGGGAGTGAGCTGGCCAGGGTCACATGGAAGTTGCCGAGAACAAGAAGGAC
AGAGACAAAGAGGCCCAGGGAAGCCAACCTGGGGACTTTCACGGGCCAAGAAGAGACTCCTGTGGCGGTT
CTTCCTGTCTGCATTTGGTTTCTTAGGCCTGTACCATTACAGGTTCATTATTATCAGGCTCATAGAAGGC
TCCATCCCCATGGGCACCTGCCCTACAGCCATAATGCCTCTGCCGAGGGACAATTTCACAGGAGTACTGC
ACCACTGGGCCCGGCCTGAAGTCCTGACCTGTACCTCTTGGGGAGCACCAATTATTTGGGATGGCACTTT
CGACCCTCATGTAGCCCAGCAAGAGGCGAGACGGCGGAACCTCACCATCGGGCTGACTGTGTTTGCTGTA
GGCAGGTACCTGGAGAAGTACCTGGAACACTTCCTGGTATCGGCAGAGCAGCACTTCATGGTCGGCCAGA
ACGTGGTGTACTATGTGTTTACGGATCGCCCGGAAGCAGTGCCCTATGTGGCTCTAGGCCAGGGTCGCCT
GCTGCGGGCAAAACCCGTGCAGCGAGAGAGGCGCTGGCAGGACGTGTCCATGGCACGCATGCCCACGCTA
CACGAGGCTCTGGGAGGGCAGCTGGGCCAAGAAGCTGACTTTGTGTTCTGCCTGGACGTGGACCAGTACT
TCACCGGTAACTTCGGGCCTGAGGTGCTGGCAGATTTGGTGGCACAGCTGCACGCCTGGCACTACCGCTG
GCCGCGGTGGCTGCTGCCCTACGAGAGGGACAAGCGATCGGCTGCTGCGCTGTCGTTAAGCGAAGGCGAT
TTCTACTACCACGCTGCGGTGTTTGGCGGCAGTGTGGCTGCACTGCTCAAGCTGACGGCCCACTGTGCGA
CTGGCCAACAGCTGGACCATAAGCGCGGCATTGAGGCACTCTGGCACGACGAAAGCCACCTTAACAAGTT
CTTCTGGCTGAACAAGCCCACCAAGCTGCTGTCGCCTGAGTTCTGCTGGGCAGAGGAAATTATCTGGAGG
AGAGAGATCCATCACCCACGCCTGCTCTGGGCACCCAAGGAATATACGCTGGTGCGAAACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001009819
Insert Size 1113 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001009819.2, NP_001009819.1
RefSeq Size 3390 bp
RefSeq ORF 1113 bp
Locus ID 215493
UniProt ID Q3V1N9
Gene Summary Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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