Sgms2 (NM_028943) Mouse Untagged Clone

CAT#: MC211528

Sgms2 (untagged) - Mouse sphingomyelin synthase 2 (Sgms2), (10ug)



  "NM_028943" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4933405A16Rik; 5133401H06Rik; AI854299
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211528 representing NM_028943
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATCATAGAGACAGCAAAACTTGAAGGTCACTTGGAAAGTCAAACCAACGACTCCACCAACACTT
ACACAAGCCCCACCGAAGCTGTAGAGGAAGAGGGCAAAAATGGCAAGGGCAAACCCAAGACCTTATCCAA
CGGGTTACGAAAGGGTGCCAAAAAATACCCGGACTACATCCAGATTTCCATGCCCAACGACTCCAAGAAC
AAGTTTCCCCTGGAGTGGTGGAAAACAGGCATCGCCTTTGTGTATGCGCTCTTCAACCTCATCCTGACAA
CCGTCATGATCACCGTCGTGCACGAGAGGGTCCCTCCCAAGGAGCTCAGCCCTCCGCTCCCAGACAAGTT
TTTTGATTACTTCGACCGGGTCAAATGGGCATTTTCTGTATCAGAAATAAACGGGATGGTATTGGTTGGG
TTATGGATCACCCAGTGGCTCTTTCTGCGTTACAAGTCAATAGTGGGGCGCAGATTCTTCTTCATCATGG
GAACTTTATACCTGTATCGCTGTATAACTATGTATGTCACTACGTTACCTGTGCCCGGAATGCACTTCCA
GTGTGCTCCCAAGCTCAATGGAGACTCTCAGGCAAAAATACAGCGGATTCTCCGGCTGATTTCTGGCGGG
GGACTGTCTATCACGGGATCTCACATCCTGTGCGGAGACTTCCTCTTCAGCGGCCACACTGTCGTGCTCA
CACTTACTTACTTGTTCATCAAAGAATATTCACCTCGTCACTTCTGGTGGTATCACTTGGTCTGCTGGCT
GCTGAGTGCGGCTGGGATCATCTGCATTCTCGTAGCGCATGAACACTATACCGTGGACGTCATCATTGCT
TACTATATCACAACACGGCTGTTTTGGTGGTACCATTCCATGGCCAACGAAAAGAACTTGAAGGTCTCTT
CCCAGACGAACTTCTTGTCTCGGGCTTGGTGGTTCCCCATCTTCTACTTTTTTGAGAAGAATGTGCAAGG
CTCAATTCCTTGCTGCTTTTCCTGGCCGCTGTCCTGGCCCCCTGGCTGCTTCAAGTCATCGTGCAGAAAG
TATTCCCGGGTCCAGAAGATCGGGGAGGATAATGAGAAGTCTACCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028943
Insert Size 1098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028943.5, NP_083219.2
RefSeq Size 5791 bp
RefSeq ORF 1098 bp
Locus ID 74442
UniProt ID Q9D4B1
Gene Summary Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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