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Spo11 (NM_001083960) Mouse Untagged Clone
CAT＃: MC209756
Spo11 (untagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 2, (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI449549; Spo11a; Spo11b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209756 representing NM_001083960
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCGCGCCTATGGGGCCCGAGGCCTCGTTCTTCGACGCCCTGGATCGGCACAGGGCTTCCCTGT TGGCCATGGTGAAGAGAGGCGCAGGGGAGACCCCTGCCGGGGCCACCCGCGTGGCCTCTAGGTTTGATGA TTCTGTCGGCCTTCGGATGATACCTCAGTGTACCACAAGAAAAATCAGAAGCGATTCACCAAAATCAGTT AAGAAATTCGCTCTGATTCTGAAAGTATTGTCCATGATTTATAAATTAATACAGAGCGACACTTATGCAA CCAAGAGAGACATATACTACACTGACAGCCAGCTCTTTGGGAACCAGGCTGCGGTGGACAGCGCCATCGA TGACATTTCCTGTATGCTGAAAGTGCCCAGGAGGAGTCTGCACGTGCTATCTACTTCCAAGGGATTGATT GCTGGCAACTTGAGATACATGGAGGAAGATGGTACCAGAGTCCAGTGTACCTGTAGTGCCACGGCTACTG CTGTGCCGACTAACATTCAAGGAATGCAGCATCTGATCACAGATGCGAAGTTTCTGTTAATAGTCGAGAA GGATGCAACATTTCAGCGGCTCCTGGACGACAACTTCTGCAGCAGGATGTCCCCGTGCATCATGGTTACG GGAAAGGGCGTTCCAGATCTGAACACGAGGCTCTTGGTAAAGAAGCTGTGGGACACCTTTCATATTCCTG TTTTCACACTGGTCGATGCAGATCCCTACGGCATCGAGATAATGTGCATCTATAAGTACGGATCCATGTC CATGTCTTTTGAAGCTCACAATCTCACTATCCCAACAATCAGATGGCTTGGTCTCCTCCCTTCTGATATC CAGAGGTTAAATATACCTAAGGATAGTTTGATCCCACTGACAAAGCATGACCAGATGAAGCTGGACAGCA TCCTGAAGAGGCCTTACATTACCTACCAGCCCCTCTGGAAAAAAGAGCTGGAGATGATGGCAGACTCTAA GATGAAGGCAGAGATCCAAGCCTTGACCTTGCTGTCGTCAGACTACCTGTCCAGAGTGTACTTACCCAAC AAGCTGAGGTTTGGAGGATGGATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001083960 |
| Insert Size | 1077 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083960.1, NP_001077429.1 |
| RefSeq Size | 1602 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 26972 |
| UniProt ID | Q9WTK8 |
| Gene Summary | Isoform 1: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226171 | Spo11 (tGFP-tagged) - Mouse sporulation protein meiosis-specific SPO11 homolog (S. cerevisiae) (Spo11) transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MR226171 | Spo11 (Myc-DDK-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 2 |
CNY 3,610.00 |
|
| MR226171L3 | Lenti ORF clone of Spo11 (Myc-DDK-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 2 |
CNY 5,510.00 |
|
| MR226171L4 | Lenti ORF clone of Spo11 (mGFP-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 2 |
CNY 5,510.00 |
