Wnt7b (NM_001163634) Mouse Untagged Clone

CAT#: MC209657

Wnt7b (untagged) - Mouse wingless-related MMTV integration site 7B (Wnt7b), transcript variant 2, (10ug)



  "NM_001163634" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Wnt-7b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209657 representing NM_001163634
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCTCCTGTCGCCGCGCAGCGCGCTGGTCTCCGTCTATTGCCCGCAGATCTTTCTCCTTCTGTCCA
GCGGCAGTTACCTAGCATTGTCATCCGTGGTGGCCCTGGGAGCCAACATCATCTGCAACAAGATTCCTGG
CCTGGCCCCACGGCAGCGTGCCATCTGCCAGAGCCGACCCGATGCCATCATTGTGATCGGGGAGGGGGCG
CAGATGGGCATCGACGAGTGCCAGCACCAGTTCCGATTCGGCCGCTGGAACTGCTCCGCCCTGGGCGAGA
AGACCGTCTTCGGGCAAGAACTCCGAGTAGGGAGTCGAGAGGCTGCCTTCACCTATGCCATCACGGCGGC
GGGCGTGGCGCATGCTGTCACCGCTGCCTGCAGCCAGGGCAATCTGAGCAATTGTGGCTGTGACCGGGAG
AAGCAAGGCTACTACAACCAGGCGGAAGGCTGGAAGTGGGGGGGCTGCTCAGCGGACGTCCGCTACGGCA
TCGACTTTTCTCGTCGCTTTGTGGATGCCCGTGAGATCAAAAAGAACGCCAGGCGCCTCATGAACCTTCA
CAACAATGAGGCGGGCAGAAAGGTTCTGGAGGACCGCATGAAGCTGGAATGTAAGTGTCACGGTGTGTCA
GGCTCCTGTACCACCAAAACTTGCTGGACCACGCTACCTAAGTTCCGCGAGGTGGGCCACCTGCTCAAGG
AGAAGTACAACGCAGCGGTGCAGGTGGAGGTGGTGCGAGCCAGCCGCCTGCGCCAGCCCACCTTCCTGCG
CATCAAGCAGCTACGCAGCTACCAGAAGCCTATGGAGACGGACCTGGTGTACATCGAGAAGTCGCCCAAC
TACTGCGAGGAGGACGCGGCCACGGGCAGCGTGGGCACGCAGGGCCGTCTGTGCAACCGCACCTCGCCGG
GGGCCGACGGCTGTGACACCATGTGCTGCGGCCGCGGCTACAACACGCACCAGTACACCAAGGTGTGGCA
GTGTAACTGCAAATTCCACTGGTGTTGCTTCGTCAAGTGCAACACGTGCAGCGAGCGCACCGAGGTCTTC
ACCTGCAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001163634
Insert Size 1062 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001163634.1, NP_001157106.1
RefSeq Size 3510 bp
RefSeq ORF 1062 bp
Locus ID 22422
UniProt ID P28047
Gene Summary Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619, PubMed:28803732). Required for normal fusion of the chorion and the allantois during placenta development (PubMed:11543617). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:28803732).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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