Wnt7a (NM_009527) Mouse Untagged Clone

CAT#: MC209655

Wnt7a (untagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a), (10ug)



  "NM_009527" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI849442; px; tw; Wnt-7a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209655 representing NM_009527
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCGGAAAGCGCGGCGCTGCCTGGGCCACCTCTTTCTCAGCCTGGGCATAGTCTACCTCCGGATCG
GTGGCTTCTCTTCGGTGGTAGCTCTGGGTGCGAGCATCATCTGTAACAAGATCCCAGGCCTGGCTCCCAG
ACAGCGGGCAATCTGCCAGAGCCGGCCGGACGCCATCATCGTCATAGGAGAAGGCTCCCAAATGGGCCTG
GACGAGTGTCAGTTTCAGTTCCGAAATGGCCGTTGGAACTGCTCAGCGCTGGGAGAGCGTACTGTCTTCG
GGAAGGAGCTCAAAGTGGGGAGTCGGGAGGCTGCCTTCACCTATGCGATTATCGCTGCGGGCGTGGCCCA
TGCCATCACTGCTGCCTGCACCCAGGGCAACCTGAGCGACTGTGGCTGCGACAAGGAGAAGCAAGGCCAG
TACCACCGGGACGAGGGCTGGAAGTGGGGTGGCTGCTCTGCCGACATCCGCTACGGCATCGGCTTCGCCA
AGGTCTTCGTGGATGCCCGGGAGATCAAGCAGAATGCCCGGACGCTCATGAACTTACACAATAACGAGGC
GGGTCGGAAGATCCTGGAGGAGAACATGAAGCTGGAGTGTAAGTGCCATGGTGTGTCAGGCTCCTGTACC
ACTAAGACGTGCTGGACCACACTGCCACAGTTCCGAGAGCTAGGCTACGTGCTCAAGGACAAATACAACG
AGGCCGTCCACGTGGAGCCTGTGCGTGCCAGTCGAAACAAGCGGCCCACCTTTCTGAAGATCAAGAAGCC
CCTGTCCTACCGCAAGCCCATGGACACTGACCTGGTGTATATCGAGAAGTCACCCAATTACTGTGAAGAG
GACCCAGTGACAGGCAGCGTGGGTACCCAGGGCCGAGCCTGCAATAAGACAGCCCCTCAGGCCAGTGGCT
GTGACCTCATGTGCTGTGGCCGTGGCTACAACACACACCAGTACGCCCGGGTGTGGCAGTGCAACTGCAA
ATTCCACTGGTGCTGCTACGTCAAGTGTAACACGTGCAGCGAGCGCACGGAGATGTATACGTGCAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_009527
Insert Size 1050 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC049093, AAH49093
RefSeq Size 3176 bp
RefSeq ORF 1050 bp
Locus ID 22421
UniProt ID P24383
Gene Summary Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341, PubMed:20530549, PubMed:23629626). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:7885472, PubMed:9769174, PubMed:9790192). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:28803732). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (PubMed:9790192). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (PubMed:23629626). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (PubMed:23629626). Promotes formation of synapses via its interaction with FZD5 (PubMed:20530549).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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