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Ppt1 (NM_008917) Mouse Untagged Clone
CAT＃: MC209220
Ppt1 (untagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1), (10ug)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9530043G02Rik; AA960502; C77813; CLN1; D4Ertd184e; INCL; PPT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209220 representing NM_008917
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGTCCTGTTCGCGGAGGCTGCTAGCTGCCGCTCTGCTGCCTTGGTGCTGCGCCGCCTGGGCCC TGGGGCATCTGGACCCACCTTCACCACCACCGCTGGTGATCTGGCATGGGATGGGGGACAGCTGTTGTAA CCCCATGAGCATGGGTGTCATTAAAAAGATGGTTGAAAAAGAAATACCTGGGATTTACGTCCTGTCTCTA GAGATTGGGAAGAACATGATGGAGGATGTGGAGAACAGCTTCTTCTTGAATGTTAATGTCCAAGTCAACA TGGTGTGTCAGATTCTGGAAAAGGATCCTAAGTTGCAGCAGGGATACAATGCTATTGGCTTCTCCCAGGG AGGCCAGTTCCTGAGGGCAGTGGCTCAGAGATGCCCAACACCTCCCATGATGACCCTGATCTCAGTTGGA GGACAACATCAAGGTGTCTTTGGACTCCCCCGATGCCCAGGAGAGAGTTCTCACATCTGCGACTTCATCA GGAAGTCACTTAATGCCGGTGCTTACTCCAAACTTGTGCAAGAACGCCTGGTGCAAGCACAGTACTGGCA TGACCCTATCAAGGAGAGTGTGTACCGAAACTACAGCATCTTCTTGGCAGACATAAATCAAGAGAGGTGT GTCAATGAGTCCTACAAGAAGAACCTGATGGCCCTCAAGAAGTTTGTGATGGTGAAATTCTTTAATGATT CCATTGTGGACCCTGTCGACTCTGAGTGGTTTGGATTTTACAGAAGTGGCCAAGCTAAGGAAACCATTCC CCTCCAGGAGAGCACTCTATACACAGAGGACCGCCTGGGGCTAAAGAAAATGGACAAAGCAGGAAAGCTA GTGTTTCTGGCTAAGGAAGGGGACCATCTTCAAATATCTAAAGAATGGTTTACTGCCCACATCATACCTT TTCTTAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008917 |
| Insert Size | 921 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008917.2, NP_032943.2 |
| RefSeq Size | 2478 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 19063 |
| UniProt ID | O88531 |
| Gene Summary | Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218476 | Ppt1 (tGFP-tagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1), (10ug) |
CNY 5,440.00 |
|
| MR218476 | Ppt1 (Myc-DDK-tagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1) |
CNY 3,840.00 |
|
| MR218476L3 | Lenti ORF clone of Ppt1 (Myc-DDK-tagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1) |
CNY 5,890.00 |
|
| MR218476L4 | Lenti ORF clone of Ppt1 (mGFP-tagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1) |
CNY 5,890.00 |
