Prkacb (NM_011100) Mouse Untagged Clone

CAT#: MC209176

Prkacb (untagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 1, (10ug)



  "NM_011100" in other vectors (6)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
PRKACB Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CbPKA; Pkacb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209176 representing NM_011100
Red=Cloning site Blue=ORF Orange=Stop codon

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGGGAACACTGCGATCGCCAAGAAAGGCAGCGAAGTGGAGAGCGTGAAAGAGTTTCTAGCCAAAGCCA
AAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGAGTAATGCTGGGCTTGAGGATTTTGAGAGGAA
GAAAACCCTCGGGACGGGTTCCTTTGGAAGAGTCATGTTGGTGAAGCATAAAGCCACTGAGCAGTACTAC
GCCATGAAGATCTTAGACAAGCAGAAGGTTGTTAAGCTGAAGCAAATAGAGCACACTCTGAATGAGAAGA
GAATCCTGCAGGCCGTGGAGTTCCCGTTCCTTGTGCGGCTGGAGTACTCTTTTAAGGATAATTCTAATTT
ATACATGGTTATGGAATACGTCCCTGGGGGAGAGATGTTCTCACATCTGAGAAGAATTGGAAGGTTCAGT
GAGCCCCACGCCCGTTTCTATGCAGCCCAGATTGTGCTAACATTTGAGTACCTTCATTCCCTCGACCTCA
TCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACCACCAGGGTTACATCCAGGTCACAGATTTCGG
GTTCGCCAAAAGAGTCAAGGGCAGGACATGGACATTGTGTGGCACCCCAGAGTACCTGGCCCCGGAGATC
ATCCTCAGCAAGGGTTACAATAAGGCGGTGGACTGGTGGGCACTGGGCGTGCTGATCTATGAGATGGCTG
CTGGCTACCCTCCATTCTTTGCTGACCAGCCAATTCAGATCTATGAGAAGATTGTCTCTGGAAAGGTCCG
GTTCCCATCACACTTCAGCTCCGATCTCAAGGACCTTCTGCGGAACCTGCTGCAGGTGGATCTGACAAAG
CGATTCGGGAACCTGAAGAACGGCGTGAGTGACATAAAGACCCACAAGTGGTTTGCCACAACTGACTGGA
TTGCTATTTATCAGAGAAAGGTTGAGGCTCCATTCATACCAAAGTTCAGAGGCTCTGGCGATACCAGCAA
CTTCGATGACTATGAAGAAGAAGAAATCCGTGTGTCTATAACAGAAAAATGTGGAAAGGAATTTTGTGAA
TTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites AscI-MluI     
ACCN NM_011100
Insert Size 1056 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011100.4, NP_035230.1
RefSeq Size 4341 bp
RefSeq ORF 1056 bp
Locus ID 18749
UniProt ID P68181
Gene Summary Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed:9368018). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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