Nog (NM_008711) Mouse Untagged Clone

CAT#: MC209047

Nog (untagged) - Mouse noggin (Nog), (10ug)



  "NM_008711" in other vectors (6)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209047 representing NM_008711
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGCTGCCCCAGCCTGGGGGTCACCCTCTACGCCCTGGTGGTGGTCCTGGGGCTGCGGGCAGCAC
CAGCCGGCGGCCAGCACTATCTACACATCCGCCCAGCACCCAGCGACAACCTGCCCCTGGTGGACCTCAT
CGAACATCCAGACCCTATCTTTGACCCTAAGGAGAAGGATCTGAACGAGACGCTGCTGCGCTCGCTGCTC
GGGGGCCACTACGACCCGGGCTTTATGGCCACTTCGCCCCCAGAGGACCGACCCGGAGGGGGCGGGGGAC
CGGCTGGAGGTGCCGAGGACCTGGCGGAGCTGGACCAGCTGCTGCGGCAGCGGCCGTCGGGGGCCATGCC
GAGCGAGATCAAAGGGCTGGAGTTCTCCGAGGGCTTGGCCCAAGGCAAGAAACAGCGCCTGAGCAAGAAG
CTGAGGAGGAAGTTACAGATGTGGCTGTGGTCACAGACCTTCTGCCCGGTGCTGTACGCGTGGAATGACC
TAGGCAGCCGCTTTTGGCCACGCTACGTGAAGGTGGGCAGCTGCTTCAGCAAGCGCTCCTGCTCTGTGCC
CGAGGGCATGGTGTGTAAGCCATCCAAGTCTGTGCACCTCACGGTGCTGCGGTGGCGCTGTCAGCGGCGC
GGGGGTCAGCGCTGCGGCTGGATTCCCATCCAGTACCCCATCATTTCCGAGTGTAAGTGTTCCTGCTAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_008711
Insert Size 699 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC132527, AAI32528
RefSeq Size 815 bp
RefSeq ORF 699 bp
Locus ID 18121
UniProt ID P97466
Gene Summary Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (PubMed:9585504, PubMed:9603738). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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