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Cd3e (NM_007648) Mouse Untagged Clone
CAT＃: MC208245
Cd3e (untagged) - Mouse CD3 antigen, epsilon polypeptide (Cd3e), (10ug)
CNY 2,640.00
CNY 3,990.00
Product images
CNY 8,040.00
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI504783; CD3; CD3epsilon; T3e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007648 |
| Insert Size | 570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007648.4, NP_031674.1 |
| RefSeq Size | 1436 bp |
| RefSeq ORF | 570 bp |
| Locus ID | 12501 |
| UniProt ID | P22646 |
| Gene Summary | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227669 | Cd3e (tGFP-tagged) - Mouse CD3 antigen epsilon polypeptide (Cd3e), (10ug) |
CNY 2,850.00 |
|
| MR227669 | Cd3e (Myc-DDK-tagged) - Mouse CD3 antigen, epsilon polypeptide (Cd3e) |
CNY 2,400.00 |
|
| MR227669L3 | Lenti ORF clone of Cd3e (Myc-DDK-tagged) - Mouse CD3 antigen, epsilon polypeptide (Cd3e) |
CNY 4,750.00 |
|
| MR227669L4 | Lenti ORF clone of Cd3e (mGFP-tagged) - Mouse CD3 antigen, epsilon polypeptide (Cd3e) |
CNY 4,800.00 |
