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Bmp8b (NM_007559) Mouse Untagged Clone
CAT＃: MC208197
Bmp8b (untagged) - Mouse bone morphogenetic protein 8b (Bmp8b), (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | O; Op3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208197 representing NM_007559
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGCGTCCGGGACTCCTATGGCTACTGGGCCTGGCTCTGTGCGTGTTGGGCGGCGGTCACCTCT CGCATCCCCCGCACGTCTTTCCCCAGCGTCGACTAGGAGTACGCGAGCCCCGCGACATGCAGCGCGAGAT TCGGGAGGTGCTGGGGCTGCCGGGGCGGCCCCGATCCCGAGCACCGGTCGGGGCTGCCCAGCAGCCAGCG TCTGCGCCCCTCTTTATGTTGGACCTGTACCGTGCCATGACGGATGACAGTGGCGGTGGGACCCCGCAGC CTCACTTGGACCGTGCTGACCTGATTATGAGCTTTGTCAACATAGTGGAACGCGACCGTACCCTGGGCTA CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT GCTGAGTTCCGGATCTACAAAGAACCCAGTACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC CTAGGACTCCGCCTCTATGTGGAAACCGAGGATGGGCACAGCATAGATCCTGGCCTAGCTGGTCTGCTTG GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTTGGTTTCTTCAGGGCCAACCAGAGTCCTGTGCG GGCCCCTCGAACAGCAAGACCACTGAAGAAGAAGCAGCTAAATCAAATCAACCAGCTGCCGCACTCCAAC AAACACCTAGGAATCCTTGATGATGGCCACGGTTCTCACGGCAGAGAAGTTTGCCGCAGGCATGAGCTCT ATGTCAGCTTCCGTGACCTTGGCTGGCTGGACTCTGTCATTGCCCCCCAGGGCTACTCCGCCTATTACTG TGCTGGGGAGTGCATCTACCCACTGAACTCCTGTATGAACTCCACCAACCACGCCACTATGCAGGCCCTG GTACATCTGATGAAGCCAGATATCATCCCCAAGGTGTGCTGTGTGCCTACTGAGCTGAGTGCCATTTCTC TGCTCTACTATGATAGAAACAATAATGTCATCCTGCGCAGGGAGCGCAACATGGTAGTCCAGGCCTGTGG CTGCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007559 |
| Insert Size | 1200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC137890, AAI37891 |
| RefSeq Size | 2940 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 12164 |
| UniProt ID | P55105 |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein may play a role in the generation of primordial germ cells, and has been shown to stimulate thermogenesis in brown adipose tissue. Male mice lacking a functional copy of this gene exhibit variable degrees of germ-cell deficiency. Homozygous knockout mice of both sexes exhibit impaired thermogenesis and reduced metabolic rate, resulting in weight gain. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217574 | Bmp8b (tGFP-tagged) - Mouse bone morphogenetic protein 8b (Bmp8b), (10ug) |
CNY 7,088.00 |
|
| MR217574 | Bmp8b (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8b (Bmp8b) |
CNY 5,488.00 |
|
| MR217574L3 | Lenti ORF clone of Bmp8b (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8b (Bmp8b) |
CNY 5,890.00 |
|
| MR217574L4 | Lenti ORF clone of Bmp8b (mGFP-tagged) - Mouse bone morphogenetic protein 8b (Bmp8b) |
CNY 5,890.00 |
