Atp5f1 (NM_009725) Mouse Untagged Clone

CAT#: MC208165

Atp5f1 (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 (Atp5f1), nuclear gene encoding mitochondrial protein, (10ug)



  "NM_009725" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-Atp5f1 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C76477
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208165 representing NM_009725
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCCCGGGTGGTGCTTTCTGCTGCCGCCACAGCGGCCCCGTGTCTGAAGAACGCGGCCGCCCTAG
GTCCAGGGGTATTACAGGCAACAAGGGCCTTTCACACAGGACAGCCTCGCCTTGCCCCTCTACCACCTCT
TCCTGAATATGGAGGAAAAGTGCGTCTTGGGCTGATTCCTGAGGAATTTTTCCAGTTCCTTTACCCTAAG
ACTGGTGTAACAGGACCTTATGTGCTTGGAACTGGACTTAGCTTGTATTTTCTATCCAAAGAAATATATG
TGATTACCCCAGAGACCTTCTCTACCATATCAGTAGTAGGGTTGATAGTCTATGTGATTAAGAAATATGG
CGCCTCTTTTGGAGAATTTATTGACAAACTTAATGAGGAAAAAATTGCTCAACTAGAAGAAGTAAAGCAG
TCGAGCATGAAACAAATCCAGGATGCAATCGACATGGAGAAGGCACAGCAGGCACTGGTTCAGAAGCGCC
ATTACCTCTTCGATGTGCAGAGGAATAACATTGCCCTGGCCTTGGAGGTCACTTACCGGGAACGGCTACA
TAAAGCATATAAGGAGGTAAAGAATCGCCTGGACTACCACATCTCTGTACAGAACATGATGCGTCGCAAG
GAGGAAGAACACATGATAGACTGGGTAGAAAAGCATGTGGTGAAGAGCATTTCTGTACAGCAGGAAAAGG
AGACCATTGCCAAGTGCATTGAAGATCTAAAGCTGCTTGCAAAGAAGGCTCAAGCTCAGCCAATTATGTG
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009725
Insert Size 771 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009725.4, NP_033855.2
RefSeq Size 1641 bp
RefSeq ORF 771 bp
Locus ID 11950
UniProt ID Q9CQQ7
Gene Summary Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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