Ambn (NM_009664) Mouse Untagged Clone

CAT#: MC208128

Ambn (untagged) - Mouse ameloblastin (Ambn), (10ug)



  "NM_009664" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208128 representing NM_009664
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGCATCTAAGATTCCACTTTTCAAAATGAAGGGCCTGATCCTGTTCCTGTCCCTAGTGAAAATGA
GCCTCGCCGTGCCGGCATTTCCTCAACAACCTGGGGCTCAAGGCATGGCACCTCCTGGCATGGCTAGTTT
GAGCCTTGAGACAATGAGACAGTTGGGAAGCTTGCAGGGACTCAACGCACTTTCTCAGTATTCTAGACTT
GGCTTTGGAAAAGCACTTAATAGTTTATGGTTGCACGGACTTCTCCCACCGCATAACTCTTTCCCATGGA
TAGGACCAAGGGAACATGAAACCCAGCAGCCATCCTTGCAGCCTCACCAGCCAGGACTGAAACCCTTCCT
CCAGCCCACTGCTGCAACCGGTGTCCAGGTCACACCCCAGAAGCCAGGGCCTCAGCCTCCAATGCACCCT
GGACAGCTGCCCTTGCAGGAAGGAGAGCTGATAGCACCAGATGAGCCGCAGGTGGCACCATCCGAAAACC
CACCAACACCTGAGGTACCAATAATGGATTTTGCTGATCCACAATTTCCAACCGTGTTCCAGATCGCCCG
TTCAATATCTCGGGGACCAATGGCACACAACAAAGCATCCGCTTTTTACCCAGGAATGTTTTACATGTCT
TATGGAGCAAACCAATTGAATGCTCCTGCCAGAATTGGCTTCATGAGTTCAGAAGAAATGCCTGGAGAAA
GAGGAAGTCCCATGGCCTATGGAACTCTGTTCCCAAGATTTGGAGGCTTCAGGCAAACCCTTAGGAGACT
GAATCAGAATTCACCCAAGGGAGGAGACTTTACTGTGGAAGTAGATTCCCCAGTATCTGTTACCAAAGGC
CCTGAAAAAGGAGAAGGTCCAGAAGGCTCTCCACTGCAAGAGGCCAACCCAGGCAAACGGGAAAACCCCG
CTCTCCTTTCACAAATGGCACCTGGGGCCCATGCAGGACTTCTTGCTTTCCCCAATGACCACATCCCCAG
TATGGCAAGGGGTCCTGCAGGGCAAAGACTCCTTGGAGTCACCCCTGCAGCTGCAGACCCACTGATCACC
CCTGAATTAGCAGAAGTTTATGAAACCTATGGTGCTGATGTTACCACACCCCTGGGTGATGGAGAAGCAA
CCATGGATATCACCATGTCCCCAGACACTCAGCAGCCACTGCTACCTGGAAACAAAGTGCACCAGCCCCA
GGTGCACAACGCATGGCGTTTCCAAGAGCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009664
Insert Size 1224 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009664.2, NP_033794.1
RefSeq Size 1874 bp
RefSeq ORF 1224 bp
Locus ID 11698
UniProt ID O55189
Gene Summary This gene encodes an extracellular matrix glycoprotein that is involved in the formation of dental enamel. Mice lacking the encoded protein fail to undergo normal ameloblast differentiation and develop enamel. Mice overproducing the product of this gene develop thinner and more porous enamel, with disrupted rod patterns and abnormal crystallites. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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