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Atg4a (NM_174875) Mouse Untagged Clone
CAT＃: MC208093
Atg4a (untagged) - Mouse autophagy-related 4A (yeast) (Atg4a), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI627006; Apg4a; Atg4al; Autl2; AV169859 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208093 representing NM_174875
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGTTATGTCCAAGTATGAAAACCAGATTCTTATTTTCCCTGACTACCTGGAAGAATTCCCGG ATACAGATGAGCTGGTATGGATTCTGGGGAAGCAGCACCCCCTTAAGACAGAAAAATCTAAGCTGTTGTC TGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAGTTTTCCCCGATTGGGGGAACAGGCCCTTCA TCTGATGCTGGATGGGGATGTATGCTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGCAGAC ACTTGGGAAGGGATTGGAACTGGGAGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTG TTTCCTAGATAGAAAAGACTGTTGCTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAA TCAATTGGCGAATGGTTTGGACCAAACACAGTTGCACAGGTGATAAAAAAACTCGCTTTATTTGATGAAT GGAATTCCTTGGCTGTTTATGTTTCCATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTG TGTTCTTCCTGTGGGTGCTGCTGACCCAGCTGGTGATTTTCTGACTGCATCCAATCAGAGTAGAGACACC TCTGTCCCGTGCTCAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCATAAACCAAATCA ATCCTGTGTATGTTGAAGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCTTTAGGAGGAAA GCCAAATAACGCCTATTATTTCATAGGATTCTTAGGAGATGAGCTCATTTTTTTGGACCCTCACACAACC CAGACTTTTGTTGACATTGAAGAGAGTGGACTAGTAGACGACCAGACTTTTCATTGCCTGCAGTCTCCAC AGCGGATGAGTATCCTGAACTTGGATCCTTCTGTGGCCTTGGGATTTTTCTGCAAAGAAGAGAAAGACTT TGATAATTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTGAGGATGTTTGAATTGGTTCAG AAGCACCCATCACACTGGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACAGGGGCAGAAT TCATAGAGTCGACTGAACAACTGGAGGACTTTGAGCTGGAGGAAGATTTTGAAATTCTGAGTGTAGGCTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_174875 |
| Insert Size | 1191 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_174875.3, NP_777364.3 |
| RefSeq Size | 2192 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 666468 |
| UniProt ID | Q8C9S8 |
| Gene Summary | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206212 | Atg4a (tGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) |
CNY 3,710.00 |
|
| MR206212 | Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) |
CNY 3,656.00 |
|
| MR206212L3 | Lenti ORF clone of Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) |
CNY 5,230.00 |
|
| MR206212L4 | Lenti ORF clone of Atg4a (mGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) |
CNY 5,230.00 |
