Rnase6 (NM_030098) Mouse Untagged Clone

CAT#: MC207775

Rnase6 (untagged) - Mouse ribonuclease, RNase A family, 6 (Rnase6), (10ug)



  "NM_030098" in other vectors (4)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9530043P15Rik; BB119466
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207775 representing NM_030098
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTAGACCTTCCCAGGTACCTTCCTCTCCTATTGCTGCTTGAGCTGTGGGAGCCGATGTATCTAC
TTTGCTCTCAGCCTAAGGGTCTCTCCAGGGCACACTGGTTTGAAATTCAGCACGTACAGACAAGTCGTCA
ACCATGCAACACAGCCATGCGCGGTGTCAACAATTATACCCAACACTGTAAACAGATAAACACCTTTCTG
CATGAATCTTTCCAGAATGTGGCTGCTACCTGCAGTTTGCACAATATCACCTGCAAGAACGGTCGGAAGA
ACTGCCACGAGAGCGCAGAACCTGTCAAGATGACTGACTGCTCACACACTGGAGGAGCCTATCCTAACTG
CCGCTATAGTAGTGATAAGCAATACAAGTTCTTCATTGTGGCCTGTGAACACCCAAAGAAGGAAGACCCT
CCCTACCAACTAGTGCCTGTGCACTTAGATAAGATTGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_030098
Insert Size 462 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030098.2, NP_084374.2
RefSeq Size 1072 bp
RefSeq ORF 462 bp
Locus ID 78416
UniProt ID Q9D244
Gene Summary Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:15693621). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:15693621, PubMed:25075772). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (By similarity). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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