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Ube2s (NM_133777) Mouse Untagged Clone
CAT＃: MC207770
Ube2s (untagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0910001J09Rik; 6720465F12Rik; AA409170; E2-EPF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207770 representing NM_133777
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCAATGTGGAGAATCTGCCGCCCCACATCATCCGCCTAGTGTACAAGGAGGTGACAACACTGA CGGCAGACCCACCTGATGGCATTAAAGTCTTCCCCAATGAGGAGGATCTCACAGACCTGCAGGTTACCAT CGAGGGCCCTGAAGGGACTCCCTATGCTGGAGGTCTGTTCCGTATGAAGCTCCTACTGGGGAAGGACTTC CCTGCCTCCCCACCCAAGGGCTACTTCCTGACTAAAATCTTCCACCCAAATGTGGGCCCCAATGGCGAGA TCTGTGTCAATGTGCTCAAGAGGGACTGGACGGCTGAATTGGGCATACGACATGTGCTGCTGACCATCAA GTGCCTGCTGATCCACCCTAACCCAGAGTCTGCACTCAATGAGGAGGCAGGCCGCCTGCTTTTGGAGAAT TATGAAGAGTATGCTGCCCGGGCCCGTCTGCTCACAGAAATCCATGGGGGTGCATGCAGCACCAGCAGCG GGAGGGCTGAGGCTACCCAAGACCTGGCCAGTGGGGCTTCAGCCTCCTCTGCTGACCCTATGATCCCAGG GGTCCTAGGGGGAGCTGAGGGTCCCATGGCCAAGAAACATGCAGGTGAGCGAGATAAGAAGCTGGCAGCC AAGAAAAAGTTGGACAAGAAGCGAGCACTGAGGCGACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133777 |
| Insert Size | 672 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133777.2, NP_598538.1 |
| RefSeq Size | 988 bp |
| RefSeq ORF | 672 bp |
| Locus ID | 77891 |
| UniProt ID | Q921J4 |
| Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202594 | Ube2s (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s) |
CNY 2,850.00 |
|
| MR202594 | Ube2s (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s) |
CNY 2,400.00 |
|
| MR202594L3 | Lenti ORF clone of Ube2s (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s) |
CNY 4,750.00 |
|
| MR202594L4 | Lenti ORF clone of Ube2s (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s) |
CNY 4,750.00 |
