Wdr45b (NM_025793) Mouse Untagged Clone
CAT#: MC207597
Wdr45b (untagged) - Mouse Wdr45 like (Wdr45l), (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 0610008N23Rik; AA408735; D16Bwg0193e; Wdr45l; WIPI-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC207597 representing NM_025793
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCCTGCCGTGTAACCCTCACGGCAACGGGCTGCTGTACGCCGGCTTCAACCAGGACCACGGGT GTTTTGCATGTGGAATGGAAAATGGATTCCGAGTCTATAATACCGATCCACTCAAAGAAAAAGAGAAACA AGAATTTTTAGAAGGAGGAGTTGGCCATGTGGAAATGTTATTTCGCTGTAACTATTTAGCTTTAGTTGGT GGTGGAAAAAAACCGAAATACCCTCCCAACAAAGTGATGATCTGGGATGATCTGAAGAAGAAGACAGTCA TCGAGATAGAATTCTCCACAGAGGTCAAGGCTGTCAAGTTGCGGAGAGATAGAATTGTGGTGGTTCTGGA CTCCATGATTAAGGTGTTCACGTTCACACACAATCCCCACCAGTTGCACGTCTTTGAAACCTGCTACAAC CCTAAAGGCCTCTGTGTCCTGTGTCCCAACAGTAACAACTCCCTTCTTGCCTTCCCTGGCACTCACACTG GCCACGTACAGCTTGTGGACCTGGCCAGCACTGAAAAGCCACCCGTGGACATTCCCGCACATGAGGGTGT CCTGAGCTGTATTGCTCTCAACCTGCAGGGAACAAGAATTGCAACTGCATCTGAAAAAGGGACCCTTATA AGAATATTTGATACTTCATCAGGGCATTTAATCCAGGAGCTACGAAGAGGATCTCAAGCAGCCAATATCT ATTGCATCAACTTCAATCAGGACGCTTCTCTCATCTGTGTGTCCAGTGATCATGGCACTGTGCACATTTT TGCAGCCGAAGATCCAAAAAGGAATAAACAGTCTAGTTTGGCATCAGCCAGTTTCCTTCCAAAATACTTC AGCTCCAAGTGGAGTTTTTCCAAGTTTCAGGTTCCTTCAGGCTCACCATGCATCTGTGCCTTTGGAACAG AGCCCAATGCAGTCATTGCTATCTGTGCAGATGGCAGCTACTACAAGTTCCTGTTCAGTCCGAAGGGCGA GTGTGTCCGGGACGTGTGTGCACAGTTCCTGGAGATGACCGATGACAAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_025793 |
Insert Size | 1035 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_025793.3, NP_080069.2 |
RefSeq Size | 2344 bp |
RefSeq ORF | 1035 bp |
Locus ID | 66840 |
UniProt ID | Q9CR39 |
Gene Summary | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases and is recruited at phagophore assembly sites where it regulates the elongation of nascent phagophores downstream of WIPI2. In the cellular response to starvation, may also function together with the TSC1-TSC2 complex and RB1CC1 in the inhibition of the mTORC1 signaling pathway.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG205176 | Wdr45b (tGFP-tagged) - Mouse Wdr45 like (Wdr45l) |
CNY 3,140.00 |
|
MR205176 | Wdr45b (Myc-DDK-tagged) - Mouse Wdr45 like (Wdr45l) |
CNY 3,656.00 |
|
MR205176L3 | Lenti ORF clone of Wdr45b (Myc-DDK-tagged) - Mouse Wdr45 like (Wdr45l) |
CNY 4,750.00 |
|
MR205176L4 | Lenti ORF clone of Wdr45b (mGFP-tagged) - Mouse Wdr45 like (Wdr45l) |
CNY 4,750.00 |