Got1 (NM_010324) Mouse Untagged Clone

CAT#: MC207293

Got1 (untagged) - Mouse glutamate oxaloacetate transaminase 1, soluble (Got1), (10ug)



  "NM_010324" in other vectors (5)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI789014; cAspAT; cCAT; Got-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207293 representing NM_010324
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCTCCATCAGTCTTTGCCCAGGTTCCGCAAGCTCCTCCGGTTCTGGTCTTTAAGCTCACTGCGG
ACTTCCGGGATGATCCAGATCCCCGCAAGGTTAACCTCGGCGTGGGAGCGTACCGCACAGATGAATCTCA
GCCCTGGGTTTTGCCAGTAGTGAGGAAGGTCGAACAGAAGATTGCTAACGACAACAGCCTCAACCACGAG
TACCTGCCCATCCTGGGCCTGGCAGAGTTCCGGAGCTGTGCTTCTCGCCTAGTTCTTGGGGACAACAGCC
TGGCTATCAGGGAGAATCGGGTTGGAGGGGTGCAGTCTTTGGGAGGGACAGGCGCTCTTCGGATTGGAGC
TGACTTCTTAGGGCGATGGTACAATGGTACAGATAACAAGAACACACCAATCTACGTATCATCACCAACC
TGGGAGAACCATAATGCTGTGTTTTCTGCCGCCGGTTTTAAGGACATTCGGCCCTATTGCTACTGGGATG
CGGAGAAGAGAGGACTGGACCTCCAGGGTTTCCTGAATGATCTGGAGAATGCCCCCGAGTTCTCCATCTT
TGTCCTCCATGCCTGTGCGCACAACCCAACAGGGACCGACCCGACTCCAGAGCAGTGGAAGCAGATCGCT
GCTGTCATGCAGCGCCGTTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTTGCATCTGGAGACC
TAGAGAAAGATGCGTGGGCTATTCGCTATTTTGTGTCTGAAGGCTTCGAGCTCTTCTGTGCCCAGTCCTT
CTCCAAGAACTTCGGGCTCTACAATGAGAGAGTGGGGAATCTGACCGTGGTCGGAAAAGAGTCTGACAGC
GTCCTGCGGGTCCTTTCCCAGATGGAGAAGATTGTACGAATCACCTGGTCCAATCCCCCAGCCCAAGGAG
CTCGGATTGTGGCCGCCACCCTCTCTGACCCGGAGCTCTTTAAGGAGTGGAAAGGTAACGTGAAGACAAT
GGCTGACCGGATTCTGACCATGAGATCCGAACTCAGGGCAAGACTAGAAGCTCTCAAGACCCCCGGGACT
TGGTCTCACATCACTGAGCAGATTGGAATGTTCAGTTTCACCGGCTTGAACCCCAAGCAGGTCGAGTATT
TGGTCAACGAGAAGCATATCTATCTCCTGCCGAGTGGTCGGATCAACATGTGCGGCTTGACCACCAAGAA
CCTAGATTACGTCGCTACCTCCATCCATGAAGCCGTCACCAAAATCCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010324
Insert Size 1242 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010324.2, NP_034454.2
RefSeq Size 2065 bp
RefSeq ORF 1242 bp
Locus ID 14718
UniProt ID P05201
Gene Summary Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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