Sucla2 (BC052774) Mouse Untagged Clone

CAT#: MC207074

Sucla2 (untagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419), (10ug)



  "BC052774" in other vectors (4)

CNY 3,230.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4930547K18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC052774
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAAATTCTATAATCTTTTTCTGAAGCATGATGCAACTATGGTAGAAATAAACCCAATGGTAGAAG
ATTCTGACGGAAAGGTGTTGTGCATGGATGCAAAGATCAATTTTGATTCAAATTCAGCCTACCGCCAGAA
GAAGATCTTTGATCTTCAGGACTGGAGTCAGGAAGACGAAAGGGACAAAGAAGCAGCTAATGCAGATATT
AACTACATCGGCCTCGATGGGAGCATAGGATGTCTAGTAAATGGTGCTGGCTTGGCTATGGCCACAATGG
ATATAATAAAACTACACGGAGGGACTCCAGCCAACTTTCTTGATGTTGGTGGAGGCGCCACAGTCCAGCA
AGTAACAGAAGCGTTTAAACTCATCACTTCAGATAAAAAGGTGCAGGCTATTCTGGTCAACATTTTTGGA
GGAATCATGAGATGTGATGTTATTGCACAAGGAATAGTCATGGCAGTAAAGGATTTAGAAATCAGAATAC
CTGTTGTGGTGCGGTTACAAGGTACACGAGTTGATGATGCTAAGGCACTAATAGCAGACAGTGGTCTTAA
AATCCTTGCTTGTGATGATTTGGATGAAGCTGCTAAAATGGTTGTAAAGCTCTCTGAAATAGTGACCTTA
GCCAAAGAAGCCCATGTGGATGTGAAGTTTCAATTGCCAATATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC052774
Insert Size 675 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052774, AAH52774
RefSeq Size 1180 bp
RefSeq ORF 674 bp
Locus ID 20916
Gene Summary ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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