Sucla2 (BC052774) Mouse Untagged Clone
CAT#: MC207074
Sucla2 (untagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419), (10ug)
CNY 3,230.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4930547K18Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC052774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAAATTCTATAATCTTTTTCTGAAGCATGATGCAACTATGGTAGAAATAAACCCAATGGTAGAAG ATTCTGACGGAAAGGTGTTGTGCATGGATGCAAAGATCAATTTTGATTCAAATTCAGCCTACCGCCAGAA GAAGATCTTTGATCTTCAGGACTGGAGTCAGGAAGACGAAAGGGACAAAGAAGCAGCTAATGCAGATATT AACTACATCGGCCTCGATGGGAGCATAGGATGTCTAGTAAATGGTGCTGGCTTGGCTATGGCCACAATGG ATATAATAAAACTACACGGAGGGACTCCAGCCAACTTTCTTGATGTTGGTGGAGGCGCCACAGTCCAGCA AGTAACAGAAGCGTTTAAACTCATCACTTCAGATAAAAAGGTGCAGGCTATTCTGGTCAACATTTTTGGA GGAATCATGAGATGTGATGTTATTGCACAAGGAATAGTCATGGCAGTAAAGGATTTAGAAATCAGAATAC CTGTTGTGGTGCGGTTACAAGGTACACGAGTTGATGATGCTAAGGCACTAATAGCAGACAGTGGTCTTAA AATCCTTGCTTGTGATGATTTGGATGAAGCTGCTAAAATGGTTGTAAAGCTCTCTGAAATAGTGACCTTA GCCAAAGAAGCCCATGTGGATGTGAAGTTTCAATTGCCAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC052774 |
Insert Size | 675 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC052774, AAH52774 |
RefSeq Size | 1180 bp |
RefSeq ORF | 674 bp |
Locus ID | 20916 |
Gene Summary | ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG202614 | Sucla2 (tGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 2,470.00 |
|
MR202614 | Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 2,280.00 |
|
MR202614L3 | Lenti ORF clone of Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 4,180.00 |
|
MR202614L4 | Lenti ORF clone of Sucla2 (mGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 4,180.00 |