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Atg10 (BC023455) Mouse Untagged Clone
CAT＃: MC206905
Atg10 (untagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268), (10ug)
CNY 3,230.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Agp10, APG10, Apg10p, Atg10l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC206905 representing BC023455.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAG CAGATAGGCGATGGCTGGGAATGGAGAACAGCCAAGGAATGTTCTGATGGCTACATGTGCAAAACACAG TTTCGAATAAAGAATGAGGCCTCGACACCACATGTGGGAACACCTGCCAGTGTGCTCACATGTCTCCCT ACAGAGGAGAATTTAGAGCTACCAATGGATGATTCTGAAGTGACGAGACCTGCAGCGGTGGCAGAAGTG ATTAAACATGAGTATCATGTCTTATACTCTTGTAGTTACCAAGTGCCGGTTCTTTACTTTAGGGCAAGC TTTTTAGATGGGAGACCGTTAGCTCTGGAGGATATATGGGAAGGTGTTCATGAGTGCTATAAGCCGCGG CTGCTCCAGGGACCGTGGGATACCATCACCCAGCAGGAACATCCAATACTTGGGCAACCATTTTTTGTT CTACATCCCTGCAAGACAAATGAATTCATGACTGCTGTGCTAAAGAATTCACAGAAAATCAATAGAAAT GTCAACTATATCACATCATGGCTGAGCCTCGTGGGGCCAGTGGTTGGTCTGAATTTACCTCTGAGCTAT GCTAAAGCAACATCACAATCGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | BC023455 |
| Insert Size | 648 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023455 |
| RefSeq Size | 1153 bp |
| RefSeq ORF | 647 bp |
| Locus ID | 66795 |
| MW | 24.6 kDa |
| Gene Summary | E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Adenovirus Type 5 Induces Cell Lysis through Autophagy and Autophagy-Triggered Caspase Activity
,null,
Journal of Virology
,PubMed ID 21367888
[Atg10]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202364 | Atg10 (tGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 2,850.00 |
|
| MR202364 | Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 2,400.00 |
|
| MR202364L3 | Lenti ORF clone of Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 4,750.00 |
|
| MR202364L4 | Lenti ORF clone of Atg10 (mGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) |
CNY 4,750.00 |
