Prc1 (BC005475) Mouse Untagged Clone

CAT#: MC206793

Prc1 (untagged) - Mouse protein regulator of cytokinesis 1 (cDNA clone MGC:6745 IMAGE:3592138), (10ug)



CNY 4,496.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms MGC6745
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC005475
CCGTTCTCCGTCCCGCGCGTGGCAAGTGGAGGGTCTCGATTCTGGACAGCTTGGAGCGTCCACCATGAGG AGAAGTGAGGTGCTAGCAGATGAGTCTATCACATGCCTGCAGAAAGCTCTAACTCACCTCCGGGAAATAT GGGAACTAATTGGGATTCCAGAGGAGCAGAGGCTACAAAGAACCGAGGTTGTAAAGAAGCATATAAAGGA TCTTCTGGATAGAATGATTGCTGAAGAGGAGAGCCTGAGGGAAAGGCTGCTTAAAAGCATATCCATCTGT CAGAAGGAGCTGAGTACCCTGTGCAGTGAGCTACAAGTGAAGCCATTTCAGGAAGAAAAAGATACAACCA TCTTGCAACTAGAAAAAGATCTGCGCACTCAAGTAGAATTGATGAGAAAACAGAAAAAGGAGAGAAAGCA GGAATTGAAGCTACTTCAGGAACAGGAGCAAGAACTCCGTGACATCCTCTGTATGCCGCCCTGTGATGTG GACAGCACCTCTGTCCCCACCTTAGAAGAGCTGAAGCTATTCCGACAGCGTGTGGCAACGCTGAGGGAGA CAAAGGAATCGAGGCGTGAAGAATTTGTCAACATTAAGAAACAAATCATATTGTGTATGGAAGAATTAGA ACACTCTCCAGATACAAGCTTTGAAAGAGATGTGGTGTGTGAAGATGAAAGTGCTTTTTGTTTATCACTG GAGAACATTGCAACATTACAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAGCAGAAT GTGAGGGGCTTCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAGAGAGCC TGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGATCGGTTA GAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAGTTCTGGG ACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACACAGAAAA CCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGCTCACAAGGAGCTGTTC CAAGGCGTCCAGAAGTGGGAAGAGAGCTGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAGATCCAG GTCGGTTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAAAACACT CCCTAAGCTGGAAGAGGAGTTGAAAGCACGGATTGAACAGTGGGAACAGGAGCACTCAACAGCATTTGTG GTGAATGGGCAGAAATTCATGGAGTATGTTACAGAACAGTGGGAATTACATCGGCTGGAGAAAGAGCGAG CCAAACAGGAGAGGCAACTGAAGAACAAGAAACAGACAGAGGCAGAGATGCTGTATGGCAGTACTCCCCG GACACCTAGCAAAAGACCAGGACAGACGCCCAAGAAGTCTGGCAAAATGAACACTACCACCATGTCTAGT GCTACACCCAACAGTAGCATTCGGCCTGTCTTTGGTGGATCAGTGTACCGGTCACCTATGTCTCGACTAC CCCCTTCTGGCAGCAAGTCAGTTGTCACTTCTCTGTGTTCTGGAAAGAAAACACCTCGAGCTGCCCAGCT TAGGGCCAACAAGGAGAACTTGGACCTCAATGGCAGCATCCTGAGCGGTGGGTACCCTGGCTCGACGCCC CTCCAGCACAACTGCAGCATTAAGTCTGTTGCCAGCACCTATTCTGAGTTTTCGCGAGAACTTTCAAAGG CTTCCAGATCTGATGCTACGTCTCGAATCCTCAATTCAACCAACATCCAGTCCTGAGAGTCCTTGTCCAG TTAGGCAGCTGGGGCTTCCTGTGCCTGGACTGGACTGACGGATACGTAGACAAGGAGTGACGTATACATT TCCATCAGTTTAGATGTTTGAAACTACCTTGGGCAGGTTCTGTTAACTGCACCTAACTCAGACATGAGTA GGACAGAAGGAAGCTGTCCCGGGCGGACTGAGGTCACAAAGACTTGCTTTTGATTCAAGAGAGACCTTAA AGGCTAGTTATGATAGTTAAGTACAAGTTTTAACATCTGGTAGCTAATTTTTTTCTCTACCCCATAATTC TACTTTGACTGCTCTTCTAGAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTA TAATGGGATCTGATGTCCTCTTCTGGTGTGCAGAAATAATTTTTTTTTAATTCTACTTATTTCAATACTA TATATTAGAAAATGAAAAAACGATTGTGGCCAGCTTTGGCATACAGGGGTACAAGACATGAAGTAATTCT GGTAACATCTAAAAATATATATATTTTTGTTCGCGGACATTTGATTAATGCTGTAGCTATGGAAGCTTTG ATGTCACCCAGAGGCGTGTACCTGGCTGGCACTATCCCAGTGAGGGTTTCTTGAGTTCCAGTATGAGATG GCCTGACCTGCTGCCAAAAAGAGTAGCAGTCATTTCTGTAGTGCAATTTCTTGAGGTATATGTGTGAACA CACATGCTTGTCTGACCTGTTGAGTTTTTCTCCAGTCTCCCAACCCTGTGCTCATTAGGCCCAGTCTGTT CCCTGTAGATGACAACCACATAGAGAGGCTGTCTGGTGGGCTGGGCCATTCCTGTCATGGAGTCAGCAAC TTTGCACATCTACAAGTTATCTGTGTAATCACGTCCACAATGAGGCTCTCCAAACCCATCACTACTCTTC CCCAAAGCACTATATTTATTCTGTCTGTCTGTGCCAGTTCTGCCCACACTGACTGATCTTGAAAACTTGT CAGTCTGCTCTTCACACACACATATATATATGTTGTTTTTAAATGTATGAAAATAAAATGTGTATAGTTA CCTTTTAAATACTAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites EcoRI-NotI     
ACCN BC005475
Insert Size 1812 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005475, AAH05475
RefSeq Size 2978 bp
RefSeq ORF 1812 bp
Locus ID 233406
Gene Summary Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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