Rab22a (BC006596) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3732413A17Rik; AI662177; AW319644; AW550514; E130120E14Rik; Rab22
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC006596, the custom clone sequence may differ by one or more nucleotides ATGGCGCTGAGGGAACTTAAAGTGTGCCTGCTCGGGGATACGGGTGTGGGTAAATCGAGCATCGTGTGGC GGTTTGTGGAAGACAGTTTTGATCCGAATATCAATCCAACCATAGGGGCATCTTTTATGACCAAGACTGT CCAGTACCAAAATGAGCTACATAAATTCCTAATCTGGGATACAGCTGGACAAGAACGATTTCGTGCATTG GCACCAATGTACTATCGAGGGTCAGCAGCAGCCATCATCGTTTACGACATCACAAAAGAAGAGACGTTTT CAACTCTGAAGAACTGGGTGAGAGAGCTCCGCCAGCACGGCCCCCCCAGCATTGTCGTTGCCATCGCAGG GAACAAGTGCGATCTTACTGATGTCAGAGAAGTCATGGAGAGAGATGCTAAGGACTACGCAGATTCCATT CATGCCATCTTTGTAGAGACCAGCGCAAAAAATGCAATAAACATAAATGAACTCTTTATAGAAATTAGTC GAAGAATTCCATCCACTGATGCCAACCCAGCGTCTGGCGGCAAGGGCTTCAAACTCCGAAGACAGCCATC GGAGCCAAAGCGAAGCTGCTGCTGA
Restriction Sites	RsrII-NotI
ACCN	BC006596
Insert Size	585 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006596, AAH06596
RefSeq Size	1911 bp
RefSeq ORF	585 bp
Locus ID	19334
Gene Summary	Plays a role in endocytosis and intracellular protein transport (PubMed:19759177, PubMed:27718357). Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth. Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form (By similarity).[UniProtKB/Swiss-Prot Function]

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