Mlkl (BC023755) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130019I15Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC023755 CCACGCGTCCGGTCGGACAGGTTGGTTTTGAGGACTCTGCTGGAGCATTGCTGCGTTGGCCCAAATTTGA CTTTAGGCGGGAACCGCGGCTGGTGCGGAGTTCCTCCGAGAGGCGAGAGCTTTAAGGTTGCACCAAGGGA GGGGACTCAGGACACCGGGGACCTGCTCCGACCTGGCGTGCATTGTCAACGAGGCAGAAGACCGCGGTCC GGGAGCGGCGGGAGACTGATGATCCTACAGGAACTGAGCGCAGGATAGACCGGACGAGCGCCCCTCGGAG ATCACGTAGAATCCAGTGAGCTGTAGCGCGGCAGGAGGAAGCAGGAGAATCCCAGAATCACAGATTGGAC TTCCTGGAACTTAGGCTATGGATAAATTGGGACAGATCATCAAGTTAGGCCAGCTCATCTATGAACAGTG TGAAAAGATGAAATACTGCCGGAAACAATGCCAGCGTCTAGGAAACCGTGTGCACGGCCTGCTACAGCCT CTCCAGAGACTCCAGGCCCAAGGAAAGAAGAACCTGCCCGATGACATTACTGCTGCCCTGGGCCGTTTTG ATGAAGTCCTGAAGGAGGCTAACCAGCAGATAGAAAAGTTCAGCAAGAAGTCCCATATTTGGAAGTTTGT GAGTGTGGGCAATGATAAGATCCTCTTCCATGAAGTGAATGAGAAGCTGAGAGACGTCTGGGAGGAGCTG TTGCTGCTGCTTCAGGTTTATCATTGGAATACCGTTTCAGATGTCAGCCAGCCAGCATCCTGGCAGCAGG AAGATCGACAGGATGCAGAGGAAGACGGAAATGAAAATATGAAAGTTATCCTGATGCAGTTGCAAATTAG CGTGGAAGAAATCAACAAAACCCTGAAGCAATGCTCACTAAAACCCACACAGGAGATCCCACAAGATCTC CAAATCAAGGAGATTCCAAAGGAACATCTTGGACCTCCGTGGACCAAACTGAAGACAAGTAAAATGAGCA CCATTTATAGAGGAGAGTATCACAGATCTCCAGTTACCATCAAAGTATTCAACAACCCCCAGGCCGAAAG TGTTGGAATAGTGAGGTTCACTTTCAATGACGAGATCAAAACCATGAAGAAATTCGATTCTCCCAACATC TTGCGTATATTTGGGATTTGCATTGATCAAACAGTGAAGCCCCCTGAGTTCTCCATTGTCATGGAGTACT GTGAACTTGGAACCCTGAGGGAACTGCTGGATAGAGAAAAAGACCTCACAATGAGTGTGCGCAGCCTCCT AGTCCTGAGGGCAGCCAGAGGCTTATACAGGCTACACCATTCGGAAACACTCCACAGAAACATCAGCAGC TCCAGTTTCCTCGTAGCCGGAGGCTACCAAGTAAAGCTTGCAGGATTTGAGTTAAGCAAAACACAGAATT CCATCAGCCGGACAGCAAAGAGCACTAAAGCAGAGAGATCCAGTTCAACGATATATGTCTCCCCTGAGAG ACTGAAAAATCCATTTTGCCTTTATGACATAAAAGCTGAAATATATAGCTTTGGAATTGTACTCTGGGAA ATTGCCACTGGAAAGATCCCATTTGAAGGCTGTGATTCTAAGAAGATCCGGGAGCTGGTGGCTGAGGACA AGAAGCAGGAACCAGTGGGTCAGGATTGCCCTGAGTTGTTGCGGGAAATCATTAATGAGTGTCGTGCCCA TGAGCCCTCCCAACGGCCCTCTGTGGACGGAATCTTGGAGAGACTGTCTGCGGTTGAAGAATCCACGGAC AAGAAGGTGTAAGAGGACAAAGTGGACATTTGTCCTTCATATCTGTTAGCAGCATCAACTTATTGCTGGG AGCAAATAGCTTCTTTCTCCCTTGCTTGCTTGCTTTTTTTCTTTCTTTTACTTGAGATGGGATCTCACCA TGTAGCCCCTGAAAACTGTGTAGAACAGGCTGGCTCAAACTCAAGGAAATCTTCCAGTTCTGCCTCCTGA ATGCTGGAATTAAAGGCATAGACCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC023755
Insert Size	1395 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023755, AAH23755
RefSeq Size	2020 bp
RefSeq ORF	1395 bp
Locus ID	74568
Gene Summary	This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

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