Hspd1 (BC016400) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	60kDa; Hsp60
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC016400 GGCCTGCCTCGCCTCGTGCTCTCCTGCCGCCGCCCCGCAGAAATGCTTCGACTACCCACAGTCCTTCGCC AGATGAGACCAGTGTCCCGGGCACTGGCTCCTCATCTCACTCGGGCCTATGCCAAAGATGTAAAATTTGG TGCGGACGCTCGAGCCTTAATGCTTCAAGGTGTAGACCTTTTAGCAGATGCTGTAGCTGTTACAATGGGG CCAAAGGGAAGAACTGTGATTATTGAACAGAGTTGGGGAAGTCCCAAAGTAACAAAAGATGGGGTCACTG TTGCAAAGTCAATTGATTTAAAGGATAAATACAAAAATATTGGAGCTAAACTTGTTCAGGACGTTGCCAA TAACACAAACGAAGAGGCTGGGGATGGCACCACCACTGCCACTGTTCTGGCACGATCTATTGCCAAGGAG GGCTTTGAGAAGATCAGCAAAGGGGCTAATCCAGTGGAAATCCGGAGAGGTGTGATGTTGGCTGTGGATG CTGTAATTGCTGAACTTAAGAAACAGTCTAAACCTGTGACAACCCCTGAAGAAATTGCTCAGGTTGCTAC AATTTCTGCAAATGGAGACAAAGACATTGGGAACATCATTTCTGATGCAATGAAAAAGGTTGGAAGAAAG GGTGTCATCACAGTGAAGGATGGAAAAACCCTGAATGATGAGCTAGAAATTATTGAAGGCATGAAGTTTG ATAGAGGATATATTTCCCCGTATTTTATTAACACATCAAAAGGTCAAAAGTGTGAATTCCAAGATGCCTA TGTCTTGTTGAGTGAAAAGAAAATTTCCAGTGTTCAGTCCATTGTCCCTGCTCTTGAAATTGCTAATGCT CATCGGAAGCCATTGGTCATAATCGCCGAAGACGTTGACGGAGAAGCTCTAAGCACGCTGGTTTTGAACA GGCTAAAAGTTGGTCTTCAGGTTGTGGCAGTCAAAGCTCCAGGATTTGGGGACAATAGGAAGAACCAGCT TAAAGATATGGCTATTGCTACTGGTGGTGCAGTGTTTGGAGAAGAGGGGTTGAATCTAAATCTTGAAGAT GTTCAAGCTCATGACTTAGGAAAAGTTGGGGAGGTCATTGTCACCAAAGATGATGCCATGCTTTTGAAAG GAAAAGGTGACAAAGCTCACATTGAAAAACGTATTCAAGAAATCACTGAGCAGCTAGACATCACAACTAG TGAATATGAAAAAGAAAAGCTGAACGAGCGACTTGCTAAACTTTCAGATGGAGTAGCTGTGTTGAAGGTT GGAGGAACAAGTGATGTTGAAGTGAATGAGAAAAAAGACAGAGTTACTGATGCTCTCAATGCTACAAGAG CAGCTGTTGAAGAAGGCATTGTTCTAGGAGGGGGCTGCGCTCTGCTTCGGTGCATCCCAGCCTTGGATTC ATTAAAGCCTGCTAATGAAGACCAGAAAATAGGTATAGAAATTATTAAAAGAGCACTTAAAATTCCTGCA ATGACGATTGCTAAGAATGCAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTCTGCAGAGTTCCTCAG AAGTTGGTTATGACGCCATGCTTGGAGATTTTGTGAACATGGTGGAAAAAGGGATCATTGATCCAACAAA GGTTGTGAGAACTGCCTTACTGGATGCTGCTGGGGTGGCCTCCTTGCTAACTACAGCCGAAGCTGTAGTG ACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCAATGGGTGGCATGGGAGGGGGTATGGGAG GCGGCATGTTCTAACTCCTAGAGTAGTGCTTTGCCCTTATCAATGAACTGTGACAGGAAGCTCAAGGCAG GTTCCTCACCAATAACTTCAGAGAAGTCACCTGGAGAAAATGACTGAAGAGAAGGCTGGCTGACCACTGT AATCATCAGTTACTGGTTTCCTTTGACGATATATAATGGTTTACTGCTGTCATTGTCCATGCCTACAGAT AATTTATTTTGTATTTTTGAATAAAGACATTTGTACATTCCTGATGCTGGGTGCAAGAGCCATATACCAG TGTCCTGCTTTCAACTTAAATCACTGAGGCATCTCTACTGTTCTGTGAGCATCAGGACTGTAGCGCTGTG TCACCACATGAGAGTTCAGAAGCAGCCTTTCTGTGGAGGGTGGGAATGATTGTGTACAAAGTAGAGAAGT ATCCAATTATGTGACAACCTTTGTGTAATAAAATTTTGTTTAAAGTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC016400
Insert Size	1722 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016400, AAH16400
RefSeq Size	2232 bp
RefSeq ORF	1722 bp
Locus ID	15510
Gene Summary	Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.[UniProtKB/Swiss-Prot Function]

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